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Hyperlinked reference study notes on the Molecular Biology of Aging
[Links,
Books,
Papers,
Links/ref,
Books/ref,
Papers/ref]
| Mechanisms of Aging
| Innovita
|[ Wikipedia
| Wikigenes
| iHOP
| SA Biosciences
]| Geron
A,
A',
A'',
B
| TA Sciences
| Sierra Sciences
| Maximum Telomere Support
| RevGenetics
| Terraternal
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Highlight Links
i. Introduction
[Text/Introduction,
____Terraternal - Live Forever. An introduction to the associated medicine.
____International Anti-Aging Systems,
Theories of Aging,
____Life Extension Magazine,
____Geron,
TA Sciences,
Medicinal Nutraceutics/Maximum Telomere Support
Sierra Sciences,
RevGenetics,
Iron-Dragon,
Biologix Peptide].
____Biological Clocks of the Aging Cell
________[LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Circadian rhythm,
(7) Telomere Clock,
Hayflick Limit].
0. Genetic Theory of Aging
________[LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Gene,
Wikipedia/Senescence;
Links/Genes in Aging,
Books].
____0.1 Genomic Instability in Premature Aging
________[Index/Genomic Stability,
LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/DNA Damage & Aging].
____0.2 Somatic Mutations and Cellular Aging
________[LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Mutations].
____0.3 Gene Silencing and Lifespan
________[Gene Silencing,
LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Gene silencing].
1. The Free Radical Theory of Aging
________[(1), Index,
Index/Oxidative Stress,
LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia].
____1.1 Oxidative DNA Damage
________[LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Oxidative stress,
Index/Oxidative Stress].
____1.2 Oxidative Damage to Proteins
________[LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Oxidative stress,
Index/Oxidative Stress].
____1.3 Mitochondrial Free Radical Production and Lipid Peroxidation
________[LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon;
Wikipedia/Lipid peroxidation,
Amazon,
Books].
____1.4 Oxidative Stress probed with Knockout and Transgenic Mice
________[LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon;
Wikipedia/Knockout mice,
Links;
Wikipedia/Transgenic mice,
Links].
2. Hormone Replacement Theory and Endocrine Models for Aging
________[(2),
Index, HGH,
IGF-1,
Estradiol,
Progesterone,
Diosgenin,
C. Racemosa (Black Cohosh),
Diosgenin,
_________LEF,
Links/Hormone Replacement Theory,
Images,
Papers,
Patents,
Books,
_________Amazon,
Wikipedia;
LEF,
Links/Endocrine Models for Aging,
Images,
Papers,
Patents,
Books,
Amazon;
Wikipedia/Endocrine system].
3. The Caloric Restriction Theory of Anti-Aging Treatment
________[(3),
Index/CR,
LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia;
________LEF/Mimicing Caloric Restriction,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia].
4. Lipofuscin Wastes and the Aging Cell Membrane
_______[(4),
Index/Lipofuscin,
LEF/Lipofuscin Treatment,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia;
________LEF/Lipofuscin Treatment,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia;
_______LEF/Cell Membrane and Aging
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Cell membrane].
____4.1 Lysosomal Degradation and Aging
[LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Lysosome].
5. The Glycation Theory of Aging
____[(5), Index/Glycation,
LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Glycation].
6. Insulin Utilization and Aging
____[(6),
Index/Insulin,
LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Insulin].
7. Hayflick Limit Theory of Aging Based on Telomeres
________[(7),
Index/Hayflick Limit,
LEF/Hayflick Limit,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Hayflick limit;
________ LEF/Telomeres and Telomerase,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Telomeres].
____7.1 Aging Damage to Telomeres
_______[Telomere Biology,
Replicative Senescence,
LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Telomere].
____7.2 Small Molecule Telomerase Activators
((7)/Telomerase Activators,
Index,
List,
lifexrefs3.html)
____________ [Index/Bioavailability,
LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon;
_____________LEF,
Links/Activate Telomerase,
Images,
Papers,
Patents,
Books,
Amazon].
________7.2.1 Geron
Telomerase Activators
(7) mostly
based on Astragalus and Ginsenosides.
(3b2/List,
US Patent 7,846,904 B2,
WO/2010/135247).
_____________[LEF,
Links/Geron Telomerase Activators,
lifexrefs3.html,
Images,
Papers,
Patents,
Books,
Amazon;
_____________Index/Astragalus,
Index/Astragalus Extract,
3b2,
LEF,
Links/Astragalus Extract,
Images,
Papers,
Patents,
Books,
Amazon;
_____________Index/Ginseng,
3b2,
LEF,
Links/Ginsenosides,
Images,
Papers,
Patents,
Books,
Amazon;
_____________Geron
A,
A',
A'',
B
| TA Sciences
| Sierra Sciences
| Maximum Telomere Support
| RevGenetics
| Terraternal].
________7.2.2 Bioavailablility of Astragalosides and Small-Molecule Telomerase Activators.
_____________[Index/Bioavailability,
LEF,
Links/Bioavailability,
Images,
Papers,
Patents,
Books,
Amazon;
_____________Links/Bioavailability of Astragalosides,
Images,
Papers,
Patents,
Books,
Amazon;
Chitosan,
Sodium Deoxycholate].
________7.2.3 DNA Nanocircles and Telomere Elongation: Eric T. Kool's Work.
Amazon].
_____________[LEF,
Links/DNA Nanocircles for Telomere Enlongation,
Images,
Papers,
Patents,
Books,
Amazon;
_____________Links/Eric T Kool Telomere Enlongation,
Images,
Papers,
Patents,
Books,
Amazon].
________7.2.4 Sierra Sciences: the hTERT Promoter and modulating hTERT expression.
_____________[LEF/William Andrews,
Sierra Sciences,
Links/Sierra Sciences,
Images,
Papers,
Patents,
Books;
_____________Links/the hTERT promoter,
Images,
Papers,
Patents,
Books,
Amazon;
_____________LEF/modulating hTERT expression,
Links,
Images,
Papers,
Patents,
Books,
Amazon].
(WO/2002/101010)
______________METHODS
AND COMPOSITIONS FOR MODULATING TELOMERASE REVERSE TRANSCRIPTASE (TERT) EXPRESSION
________7.2.5 Nitric Oxide as a Telomerase Activator.
______________[Index/Nitric Oxide,
LEF/Nitric Oxide,
NO More Heart Disease,
Links/Nitric Oxide activates telomerase,
Images,
Papers,
Patents,
Books].
________7.2.6 Novel Wistar Institute Telomerase Activators
____________[Tri-Phenyl Compounds,
Links/Wistar Institute Telomerase Activators,
Images,
Papers,
Patents,
Books].
____________Aviv Gazit et al,
TELOMERASE
ACTIVATING COMPOUNDS AND METHODS OF USE THEREOF,
____________US Patent,
US Patent App. 12/602,956, 2008,
WO Patent WO/2008/149, 353, 2008.
________7.2.7 Androgens as Small-Molecule Telomerase Activators
[List/Androgens,
Links,
Images,
Papers,
Patents;
Books/Androgens and Aging;
LEF/Androgens,
Wikipedia].
____7.3 Telomerase Inhibitors
(List).
_________[LEF/telomerase inhibitors,
Links,
Images,
Papers,
Patents,
Books,
Amazon].
____7.4 The hTERT Promoter.
(Index/hTERT Promoter)
[LEF/hTERT;
Links/The hTERT Promoter,
Images,
Papers,
Patents,
Books].
____7.5 Telomere Epigenetics - The Role of Methylation
_________[Links,
Images,
Papers,
Patents,
Books,
_________LEF/Epigenetics in Aging,
Links/Epigenetics in Aging,
Images,
Papers,
Patents,
Books,
_________LEF/Methylation in Aging,
Links,
Images,
Papers,
Patents,
Books].
____7.6 The Telomere Position Effect and Youthful Patterns of Gene Expression
_________[(7)/Telomere Position Effect,
Links,
Images,
Papers,
Patents,
Books,
_________Links/The Telomere Position Effect,
Images,
Papers,
Patents,
Books,
_________LEF/hTERT,
Links,
Images,
Papers,
Patents,
Books].
____7.7 Telomere T-Loop and D-Loop
(Index/T-Loop).
_________[Links,
Images,
Papers,
Patents,
Books,
_________LEF/The Telomere T-Loop,
Links,
Images,
Papers,
Patents,
Books,
_________Links/The Telomere D-Loop,
Images,
Papers,
Patents,
Books].
____7.8 Telomere Capping Proteins
((7)/Telomere Capping Proteins,
Index/Telosome)
_________[Links,
Images,
Papers,
Patents,
Books;
Index/Mammalian Telomere Protein Factors,
Index/The Telomere Interactome].
____7.9 Telomerase Functional Environment
(Index/Telomerase)
[lifexrefs4.html,
Links,
Images,
Papers,
Patents,
Books].
____7.10 Telomerase and Protection of Mitochondrial DNA
_________[(7)/Telomerase and Aging in Mitochondria,
Links,
Images,
Papers,
Patents,
Books,
_________LEF/Protection of Mitochondrial DNA,
Links,
Images,
Papers,
Patents,
Books].
____7.11 Measuring Telomere Length
[Lifexlabs/Telomeasures,
Links,
Images,
Papers,
Patents,
Books].
___________7.11.1 with PCR Methods (Cawthon).
[Links,
Images,
Papers,
Patents,
Books].
___________7.11.2 with Southern blot analysis of telomere restriction fragments.
[Links,
Images,
Papers,
Patents,
Books].
___________7.11.3 with Quantitative fluorescence in situ hybridization using digital fluorescence microscopy (Q-FISH).
_________________[Links,
Images,
Papers,
Patents,
Books].
___________7.11.4 with Quantitative fluorescence in situ hybridization using flow cytometry (flow-FISH).
_________________[Links,
Images,
Papers,
Patents,
Books].
___________7.11.5 with Quantitative fluorescent in situ hybridization laser scanning cytometry (Q-FISHLSC).
_________________[Links,
Images,
Papers,
Patents,
Books].
8. Stem Cell Technology and Anti-Aging Therapy
[(8),
Wikipedia/Stem cells;
LEF,
Links,
Images,
Papers,
Patents,
Books;
Wikipedia/Stem cells].
___8.1 Introduction to Stem Cells
___8.2 Induced Pluripotent Stem Cells (IPS Cells)
[Links,
Images,
Papers,
Patents,
Books].
___8.3 Further Stem Cell References.
9. Aging and Exercise
[(9),
LEF,
Links,
Images,
Papers,
Patents,
Books].
10. Aging and DNA Repair
[(10),
Wikipedia,
LEF,
Links,
Images,
Papers,
Patents,
Books].
11. The Immune System and Aging
[(11),
Wikipedia/Immune System;
LEF,
Links,
Images,
Papers,
Patents,
Books].
12. Aging and Chelation of Metallic Wastes
[(12),
Index,
LEF,
Links,
Images,
Papers,
Patents,
Books].
13. The Membrane Theory of Aging (Zs-Nagy)
[(13),
LEF,
Links,
Images,
Papers,
Patents,
Books].
14. The Proteasome and Aging
[(14),
Index/Proteasomes,
Wikipedia;
LEF,
Links,
Images,
Papers,
Patents,
Books].
15. Other Mechanisms of Aging [(15)].
____15.1 Racemization [(15)/Racemization,
Wikipedia/Racemization;
Links/in life span measurements,
________Links/in life span dating assay;
Books/racemization assay,
Papers/in life span determination].
____15.2 Smoking [(15)/Smoking,
Links,
Images,
Video,
Papers,
Books,
LifeExtension].
____15.3 History of Infections [(15)/Infections,
[Links,
Images,
Video,
Papers,
Books,
LifeExtension;
________Links/Bacterial
pathogens in human disease (Bacterial
infections),
Links/Viral pathogens
in human disease (viral infections)].
____15.4 Wear and Tear - Dental [(15)/Wear and Tear - Dental, Dental].
____15.5 Non-Oxidative Transformations of DNA and Proteins in Aging
[LEF,
Links,
Images,
Papers,
Patents,
Books].
____15.6 Dysregulation of Transcription and Translation in Aging
[Links,
Images,
Papers,
Patents,
Books].
____15.7 RNA Damage and Aging
[Wikipedia/RNA;
LEF,
Links,
Images,
Papers,
Patents,
Books].
16. Telomerase Inhibitors.
[List,
LEF,
Wikipedia,
Links,
Images,
Papers,
Patents,
Books;
High Polyphenol Diet].
17. Molecular Biology of Aging References.
18. Selected references on Astragalosides and Ginsenosides as Telomerase Activators
____[Index/Astragalosides;
3b2/Astragalosides;
Links/Astragalosides as Telomerase Activators,
Images,
Papers,
Patents,
Books;
____Links/References on Astragalosides,
Images,
Papers,
Patents,
Books;
____Index/Ginseng;
3b2/Ginsenosides;
Links/Ginsenosides as Telomerase Activators,
Images,
Papers,
Patents,
Books;
_____Links/References on Ginsenosides,
Images,
Papers,
Patents,
Books].
19. Selected References on the Characteristics of Telomerase
_____[Further Telomerase References,
Index/Telomerase;
Links/Characteristics of Telomerase,
Images,
Papers,
Patents,
Books;
_____Links/References on Characteristics of Telomerase,
Images,
Papers,
Patents,
Books].
20. Selected References on the biological activity of ginsenosides and astragalosides
_____[Index/Ginsenosides;
Links/Biological Activity of the Ginsenosides,
Images,
Papers,
Patents,
Books;
_____Links/Biological Activity of the Astragalosides,
Images,
Papers,
Patents,
Books].
21. Selected References on Enhancement of Antiviral Function using Cycloastragenol (TAT2)
_____[Index/TAT2,
Index/cycloastragenol;
Links/Enhancement of Antiviral Function,
Images,
Papers,
Patents,
Books;
_____Links/Enhancement of Antiviral Function with TAT2,
Images,
Papers,
Patents,
Books].
22. Further References on Telomerase
_____[Index/Telomerase;
Links/Characteristics of Telomerase,
Papers,
Patents,
Books;
_____Links/References on Characteristics of Telomerase,
Images,
Papers,
Patents,
Books].
23. References on Zinc Finger Nucleases
_____[Index/Zinc Finger Nucleases;
Links/Zinc Finger Nucleases,
Images,
Papers,
Patents,
Books;
_____Links/ZFN Technology,
Images,
Papers,
Patents,
Books].
24. Selected References on Hair Graying
_____[Index,
Links/Hair Graying,
Images,
Papers,
Patents,
Books,
LifeExtension;
_____Links/Cures for Grey Hair,
Images,
Papers,
Patents,
Books,
LifeExtension;
_____Links/Hair Follicle Melanocytes,
Images,
Papers,
Patents,
Books,
LifeExtension;
_____Links/Hair Follicle Melanocyte Stem Cells,
Images,
Papers,
Patents,
Books,
LifeExtension].
25. Selected References on Heat Shock Proteins
_____[Index,
Wikipedia/Heat_shock_protein,
_____Links/Heat Shock Proteins,
Images,
Papers,
Patents,
Books,
LifeExtension].
26. The Aging Cell
[Links/The Cell,
Images,
Video,
Papers,
Patents,
Books;
Links/The Aging Cell,
Images,
Video,
Papers,
Patents,
Books].
6. Insulin Utilization and Aging
____[ (6),
Index/Insulin,
Diabetes;
LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Insulin].
7. Hayflick Limit Theory of Aging Based on Telomeres
[ (7),
Links,
Images,
Papers,
Patents,
Books].
See also TA Sciences Science Library.
Jerry W. Shay and
Woodring E. Wright (2000),
Hayflick, his limit,
and cellular ageing,
Nature/Reviews/Molecular
Cell Biology, October, 2000.
Shows microphotographs of the difference between young and old human
fibroblast
[ Books] diploid cells
(strain WI-38).
Carrel, A. (1912),
On the permanent life of tissues
outside of the organism,
Journal of Experimental Medicine, 1912, 15: 516-527.
Hayflick had to contend with Nobelist Carrel concerning limits to cell division in vitro.
Harry Eagle (1955),
Nutrition
Needs of Mammalian Cells in Tissue Culture,
Science 122: 1955, pp. 501-504.
The medium developed by Eagle is still the basic medium used for animal cell culture.
H. Earle Swim and
Robert F. Parker (1957),
CULTURE CHARACTERISTICS
OF HUMAN FIBROBLASTS PROPAGATED SERIALLY,
American Journal of Epidemiology, 1957,
Volume 66, Number 2 Pp. 235-243, including 24 references with titles and historic micophotos.
[ Papers].
This paper, which includes some good microphotos of
non-senescent and
senescent cells,
seems to be one of the first to have detected limits to the division of human foreskin
fibroblasts in culture at the 49th passage,
a topic reviewed again by
Hayflick and Moorhead in 1961.
A few of Swim and Parker's
fibroblasts from 2 ( uterine
and foreskin fibroblast)
of ten strains and 51 cultures examined seem to have escaped in
morphologically
transformed and
immortalized
condition
(resembling that of
malignant cells)
as strains U12-705 and FS4-705,
still dividing after 3 years at the time of publication. Normal cultures invariably stopped propagating after
2 to 12 months of active growth. It was noted in a separate paper
( Haff and Swim, 1956)
that rabbit fibroblasts
sometimes escaped as immortal cells without morphological transformation
corresponding to a
malignant
phenotype.
Swim and Parker were looking for media that would
keep fibroblast cultures propagating indefinitely, but photographed two
young fibroblast cultures
(of uterine and foreskin fibroblasts) after a few weeks
and compared them to the
senescent fibroblasts
obtained from the same cells grown in their better media
after a year and 40 passages (uterine) or 49 passages (foreskin),
each passage corresponding to a cell number doubling.
Signs of morphological
transformations corresponding to senescent cells were especially evident in their photo of
foreskin fibroblasts at 49 cell divisions.
Foreskin fibroblasts divided more vigorously and for a longer period than fibroblasts
obtained from 10 other tissues.
Media featuring normal horse serum supported fibroblasts better than media featuring
human serum or bovine serum. Media
containing 5% beef embryo extract and 10 to 20 per cent horse serum worked best, and many different media combinations
were tested.
H. Earle Swim and Robert F. Parker worked at the Department of Microbiology,
Western Reserve University,
School of Medicine, in Cleveland, Ohio.
Leonard Hayflick and
Moorehead PS (1961),
Serial Cultivation
of human diploid cell strains,
Exp Cell Res. 25:585-621, including
35 references without titles, and no photos.
"Cell strains ( of non-transformed fibroblasts)...degenerate after about 50 subcultivations
and 1 year in culture....the phenomenon is attributable to intrinsic factors which are expressed as
senescence at the cellular level." (italics mine). Hayflick and Moorehead define
" cell strains"
as cultures of morphologically unaltered cells and
" cell lines" as
cultures of morphologically transformed cells, which may feature
heteroploid characteristics and immortalized cellular propagation.
The article emanated from the "Wistnr" Institute of Anatomy and Biology, Philadelphia, Pennsylvania. This
paper was anticipated in most respects by the prior research of
Swim and Parker in 1957.
JD Watson (1972),
Origin of concatemeric T7 DNA,
Nature New Biology,
239:197-201, 1972.
The key initial paper in the end-replication problem
[ Links,
Books,
Papers,
Images,
Amazon/the
telomere end-replication problem] involving
Okazaki fragments in
DNA lagging strand synthesis
and RNA primers.
Leads to a consideration of
incomplete
copying of template margin in enzymic synthesis of polynucleotides
as developed by Alexey Olovnikov
for application to cellular age timekeeping.
AM Olovnikov (1973),
A theory of marginotomy.
The incomplete copying of template margin in enzymic synthesis of polynucleotides and biological significance
of the phenomenon as a cellular clock.,
Journal
of Theoretical Biology, Sep 14,1973;41(1):181-90.
Elizabeth Blackburn,
Joseph G Gall
[ Links] (1978),
A
tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in Tetrahymena,
Journal of Molecular Biology
1978 Mar 25;120(1):33-53. ( Discovery of the telomere.)
Greider CW and
Blackburn EH (1985),
Identification
of a specific telomere terminal transferase activity in Tetrahymena extracts. (Discovery of telomerase),
Cell 43:405-13.
Cooke HJ,
Smith BA (1986),
Variability at the telomeres of the human X/Y pseudoautosomal region,
Cold
Spring Harbor Symposium for Quantitative Biology 1986;51 Pt 1:213-219.
Cooke and Smith reported that telomeres are longer in reproductive
haploid cells such as spermatazoa and
egg cells but shorten with age in other somatic cells.
Moyzis RK, Buckingham JM, Cram LS, Dani M, LL (1988),
A
Highly Conserved Repetitive DNA Sequence,(TTAGGG)n, present at the Telomeres of Human Chromosomes,
Proc
Natl Acad Sci. 85(18): 6622-6626.
Wright WE,
Shay JW,
Pereira-Smith OM (1989),
Reversible Cellular Senescence: implications for immortalization of normal human diploid fibroblasts,
Mol Cell Biol, 9(7): 3088-3092.
Morin, G. B. (1989),
The
human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats,
Cell 59:521-529.
CB Harley,
Futcher, and
Greider (1990),
Telomeres shorten
during aging of human fibroblasts,
Nature 345: 458-460.
Shay, J.W., Pereira-Smith, O.M., and Wright, W.E. (1991),
A
role for both Rb and p53 in the regulation of human cellular senescence,
Experimental Cell Research, 196, 33-39.
Counter CM, Avilion AA, LeFeuvre CE, Stewart NG, Greider CW, Harley CB, Baccetti S (1992),
Telomere
shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity,
EMBO Journal 1992, 11:192-1929.
Richard C. Allsopp,
H Vaziri,
C Patterson,
S. Goldstein,
E. V. Younglai,
A Bruce Futcher,
Carol W. Greider
and Calvin B. Harley (1992),
Telomere Length Predicts
Replicative Capacity of Human Fibroblasts,
Proc. Natl. Acad. Sci. USA,
vol. 89, November 1992.
Counter, C. M., A. A. Avilion, C. E. LeFeuvre, N. G. Stewart, C. W. Greider, C. B. Harley, and S. Bacchetti (1992),
Telomere
shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity,
EMBO Journal, 11:1921-1929.
West, MD (1994),
The cellular and molecular biology
of skin aging,
Archives of Dermatology,
1994 130(1) 87-95.
Richard C. Allsopp,
Edwin Chang,
Mohammad Kashefi-Aazam,
Evgeny I. Rogaev,
Mieczyslaw A. Piatyszek,
Jerry W. Shay and
Calvin B. Harley (1995),
Telomere
Shortening Is Associated with Cell Division in Vitro and in Vivo,
Experimental Cell Research,
Volume 220, Issue 1, September 1995, Pages 194-200.
RC Allsopp, E Chang, M Kashefi-Aazam, EI Rogaev, (1995),
Telomere shortening
is associated with cell division in vitro and in vivo.,
Experimental Cell Research, 1995.
Allsopp, R. C., and C. B. Harley (1995),
Evidence
for a critical telomere length in senescent human fibroblasts,
Experimental Cell Research, 219:130-136.
Scott Henderson,
Richard Allsopp,
David Spector,
Sy-Shi Wang,
Calvin Harley (1996),
In Situ Analysis of Changes in Telomere Size
during Replicative Aging and Cell Transformation,
Journal of Cell Biology,
Volume 134, 1996, pp 1-12.
Atadja, P., Wong, H., Garkavtsev, L., Veillette, C., and Riabowol, K. (1995),
Increased activity of p53 in senescing fibroblasts,
Proceedings of the National Academy of Science USA, 92, 3848-8352.
Hara, E., Tsuri, H, Sinozaki, S., and Oda, K. (1996),
Cooperative
effect of antisense-Rb and antisense-p53 oligomers on the extension of lifespan in human diploid fibroblasts,
Biochemistry and Biophysics Research Communications, 179, 528-534.
Lansdorp, P.M., Verwoerd, N.P., van de Rijke, F.M., Dragowska, V.,
Little, M.T., Dirks, R.W., Raap, A.K., and Tanke, H.J. (1996),
Heterogeneity
in telomere length of human chromosomes,
Human Molecular Genetics 5: 685-691.
Alcorta, D.A., Xiong, Y., Phelps, D., Hannon, G., Beach, D., and Barrett, J.C. (1996),
Involvement
of the cyclin-dependent kinase inhibitor p16 (INK4a) in replicative sensescence of normal human fibroblasts,
Proceedings of the National Academy of Sciences USA, 93, 13742-13747.
Wright, W.E., V.M.Tesmer, K.E.Huffman, S.D.Levene, and J.W.Shay (1997),
Normal
human chromosomes have long G-rich telomeric overhangs at one end,
Genes and Development 11: 2801-2809.
Thomas R. Cech et al, (1997),
( Geron Patent)
Telomerase, US Patent 6093809.
Calvin B. Harley (1997),
Human
Aging
and Telomeres,
Ciba Found Symposium
1997;211:129-39; discussion 139-44.
[ Papers].
CP Chiu and
CB Harley (1997),
Replicative senescence
and cell immortality: the role of telomeres and telomerase,
Proceedings
of the Society for Experimental Biology and Medicine, Vol 214, 99-106.
[ Links,
Images,
Papers,
Patents,
Books].
Serrano, M., Lin, A.W., McCurrach, M.E., Beach, D., and Lowe, S.W. (1997),
Oncogenic
ras provokes premature cell senescence associated with accumulation of p53 and p16INK4a,
Cell 88, 593-602.
[ Index/p16,
Index/p53,
Links/premature
cell senescence associated with accumulation of p16INK4a,
Images,
Papers,
Patents,
Books,
Links/premature
cell senescence associated with accumulation of p53,
Images,
Papers,
Patents,
Books].
Gire, V., and Wynford-Thomas, D. (1998),
Reinitiation
of DNA synthesis and cell division in senescent human fibroblasts by microinjection of anti-p53 antibodies,
Mol. Cell Biol. 18, 1611-1621.
Kiyono,T., Foster, S.A., Koop, J.I., McDougall, J.K., Galloway, D.A., and Klingelhutz, A.J. (1998),
Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells,
Nature 396, 84-88.
Nugent, C.I., and Lundblad, V. (1998),
The
telomerase reverse transcriptase: components and regulation,
Genes and Development 12: 1073-1085.
Andrea G. Bodnar,
Michel Ouellette,
Maria Frolkis,
Shawn E. Holt,
Choy-Pik Chiu,
Gregg B. Morin,
Calvin B. Harley,
Jerry W. Shay,
Serge Lichtsteiner,
Woodring E. Wright (1998),
Extension
of life-span by introduction of telomerase into normal human cells,
Science 16 January 1998, pp. 349 - 352.
Homayoun Vaziri and Samuel Benchimol (1998),
Reconstitution
of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span,
Current Biology 8, 5: 26 Feb 1998, 279-282.
Counter, C. M., M. Meyerson, E. N. Eaton, L. W. Ellisen, S. D. Caddle, D. A. Haber, and R. A. Weinberg (1998),
Telomerase
activity is restored in human cells by ectopic expression of hTERT (hEST2), the catalytic subunit of telomerase,
Oncogene, 16:1217-1222.
Vaziri, H., and S. Benchimol (1998),
Reconstitution
of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span,
Curr. Biol, 8:279-282.
Shawn E. Holt, Dara L. Aisner, Joseph Baur, Valerie M. Tesmer, Marife Dy, Michel Oullette, James B. Trager,
Gregg B. Morin, David O. Toft, Jerry W. Shay, Woodring E. Wright, and Michael A. White (1999),
Functional
requirement of p23 and Hsp90 in telomerase complexes,
Genes and Development 1999 13: 817-826.
Herbert, B., A.E.Pitts, S.I.Baker, S.E.Hamilton, W.E. Wright, J.W.Shay, and D.R. Corey (1999),
Inhibition
of human telomerase in immortal human cells leads to progressive telomere shortening and cell death,
Proc. Natl. Acad. Sci. USA 96:14276-14281.
Brehm, A., and Kouzarides, T. (1999),
Retinoblastoma protein meets chromatin,
Trends in Biochemical Sciences 24, 142-145.
Stein, G.H., Drullinger L.F., Soulard, A., Dulic, V. (1999),
Differential
roles for cyclin-dependent kinase inhibitors p21 and p16
in the mechanisms of senescence and differentiation in human fibroblasts,
Mol.Cell.Biol., 19, 2109-2117.
Ducray C., Pommier, J.P., Martins, L., Boussin, F.D., and Sabatier, L (1999),
Telomere
dynamics, end-to-end-fusions and telomerase activation during the human fibroblast immortalization process,
Oncogene,
18, 4211-4223.
David Stipp (1999),
The Hunt For
The Youth Pill From cell-immortalizing drugs to cloned organs,
biotech finds new ways to fight against time's toll.
Fortune, October 11, 1999.
( TA Sciences: TA-65).
Green suggests astragalus extracts, plenty,
with perseverance [ 81s].
Carmela P. Morales,
Shawn E. Holt,
Michel Ouellette,
Kiran J. Kaur,
Ying Yan,
Kathleen S. Wilson,
Michael A. White,
Woodring E. Wright &
Jerry W. Shay, (1999),
Absence of cancer−associated changes
in human fibroblasts immortalized with telomerase,
Nature Genetics
21, 115 - 118 (1999).
Griffith, Comeau, and Rosenfield (1999),
Mammalian Telomeres
end in a Large Duplex Loop,
Cell 97: 503-514.
M. A. Blasco,
Susan M. Gasser, and
Joachim Lingner, (1999),
Telomeres and telomerase,
Genes and Development,
Vol. 13, No. 18, pp. 2353-2359, September 15, 1999.
Carol W. Greider (1999),
Telomeres
Do D-Loop T-Loop Minireview.
Jerry W. Shay (1999),
On telomeric t-loops and d-loops.
Actually, this was Jerry W. Shay (1999).
At the end of the millenium, a view of the end,
Nature Genetics, 23, 382-383 (1999).
Kami Ahmada and Kent G. Golica (1999),
Telomere Loss in Somatic Cells
of Drosophila Causes Cell Cycle Arrest and Apoptosis,
Genetics, Vol. 151, 1041-1051, March 1999.
Carmela P. Morales,
Shawn E. Holt,
Michel Ouellette,
Kiran J. Kaur,
Ying Yan,
Kathleen S. Wilson,
Michael A. White,
Woodring E. Wright &
Jerry W. Shay (1999),
Absence of cancer−associated changes
in human fibroblasts immortalized with telomerase,
Nature Genetics 21, 115 - 118 (1999).
Izumi Horikawa,
P. LouAnn Cable,
Cynthia Afshari and
J. Carl Barrett (1999),
Cloning and Characterization
of the Promoter Region of Human Telomerase Reverse Transcriptase Gene,
Cancer Research,
59, 826-830, February 1, 1999
[ Papers,
Books].
Herbert, B., A. E. Pitts, S. I. Baker, S. E. Hamilton, W. E. Wright, J. W. Shay, and D. R. Corey (1999),
Inhibition
of human telomerase in immortal human cells leads to progressive telomere shortening and cell death,
Proc. Natl. Acad. Sci, USA 96:14276-14281.
Bryce LA, et al., (2000),
Mapping
the gene for human telomerase reverse transcriptase, hTERT, to chromosome 5p15.33 by
fluorescence in situ hybridization,
Neoplasia, 2000.
S. Marcand, V. Brevet, C. Mann, and E. Gilson (2000),
Cell
cycle restriction of telomere elongation,
Current Biology 10, 487-490.
WD Funk, CK Wang, DN Shelton,
CB Harley, GD Pagon, et al., (2000),
Telomerase
Expression Restores Dermal Integrity to in Vitro-Aged Fibroblasts
in a Reconstituted Skin Model,
Experimental Cell Research, Volume 258, Number 2,
August 2000 , pp. 270-278(9).
DG Farwell, KA Shera, JI Koop, GA Bonnet, et al., (2000),
Genetic
and Epigenetic Changes in Human Epithelial Cells Immortalized by Telomerase,
American Journal of Pathology,
2000;156:1537-1547.
Yong-Ouk You,
Gene Lee and
Byung-Moo Min, (2000),
Retinoic Acid
Extends the in Vitro Life Span of Normal Human Oral Keratinocytes by Decreasing p16INK4A Expression
and Maintaining Telomerase Activity,
Biochemical
and Biophysical Research Communications,
Volume 268, Issue 2, 16 February 2000, Pages 268-274.
Bailey, S.M., Cornforth, M.N., Kurimasa, A., Chen, D.J., and Goodwin, E.H., (2001),
Strand-specific postreplicative processing of mammalian telomeres,
Science 293: 2462-2465.
Baur, J.A., Zou, Y., Shay, J.W., and Wright, W.E. (2001),
Telomere
position effect in human cells,
Science 292, 2075.
J.L. Munoz-Jordan, G.A. Cross, T. de Lange, and J. D. Griffith (2001),
T-loops at typanosome telomeres,
EMBO Journal 20, 579-588.
Blackburn, Elizabeth, (2001),
Switching and signaling
at the telomere,
Cell, Vol. 106, 661-673, September 21, 2001.
Ali Ahmed, MD, MPH, and Trygve Tollefsbol, PhD (2001),
Telomeres and Telomerase:
Basic Science Implications for Aging,
Journal of the American Geriatrics Society,
Volume 49 Issue 8 Page 1105-1109, August 2001.
Campisi, J., Kim, S., Lim, C., and Rubio, M. (2001),
Cellular
senescence, cancer, and aging: The telomere connection,
Experimental Gerontology, 36, 1619-1637.
Itahana, K., Dimri, G., and Campisi, J. (2001),
Regulation
of cellular senescence by p53,
European Journal of Biochemistry, 268, 2784-2791.
Rheinwald, J.G., et al. (2002),
A
two-stage, p16(INK4a)- and p53-dependent keratinocyte senescence mechanism that
limits replicative potential independent of telomere status,
Mol. Cell. Biol., 22, 5157-5172.
N. R. Forsyth, W. E. Wright,
J. W. Shay (2002),
Telomerase
and differentiation in multicellular organisms: Turn it off, turn it on, and turn it off again,
Differentiation,
Volume 69 Issue 4-5 Page 188-197, January 2002.
Robert F. Newbold, (2002),
The significance of telomerase activation
and cellular immortalization in human cancer,
Mutagenesis,
Vol. 17, No. 6, 539-550, November 2002.
Yu-Sheng Cong, Woodring E. Wright, and
Jerry W. Shay (2002),
Human Telomerase and Its Regulation,
Microbiology
and Molecular Biology Reviews, Sept. 2002.
Karlsreder, J., Smogorzewska, A., and de Lange, T. (2002),
Senescence
induced by altered telomere state, not telomere loss,
Science 295, 446-2449.
Ithana,K., et al. (2003),
Control
of the replicative life span of human fibroblasts by p16 and the polycomb protein Bmi-1,
Mol Cell. Biol., 23, 389-401.
Nicholas R. Forsyth,
A. Patrice Evans,
Jerry W. Shay and
Woodring E. Wright (2003),
Developmental differences
in the immortalization of lung fibroblasts by telomerase,
Aging Cell: 2 (5), 235-243.
RC Allsopp, GB Morin, R DePinho,
CB Harley, (2003),
Telomerase
is required to slow telomere shortening and extend replicative lifespan of HSCs during serial transplantation,
Blood,
15 July 2003, vol. 102, no. 2. pp. 517-520. HSCs are
hemopoietic stem cells.
Silvia Di Donna,
Kamel Mamchaoui, Racquel N. Cooper, Sophie Seigneurin-Venin, Jacques Tremblay,
Gillian S. Butler-Browne and Vincent Mouly (2003),
Telomerase Can Extend the
Proliferative Capacity of Human Myoblasts, but Does Not Lead to Their Immortalization,
Molecular Cancer Research
1:643-653.
RM Cawthon,
KR Smith,
E O'Brien,
A Sivatchenko, RA, et al. (2003),
Association
Between Telomere Length in blood and mortality in people aged 60 years or older,
( direct
link to the paper)
The Lancet, 2003.
Christian M. Beausejour, Ana Krtolica, Francesco Galimi, Masashi Narita,
Scott W. Lowe, Paul Yaswen, and Judith Campisi (2003),
Reversal
of human cellular senescence: roles of the p53 and p16 pathways,
The EMBO Journal, Vol.22. No.16 pp 4212-4222, 2003.
Tony Chih Yuan Chuang, Sharareh Moshir, Yuval Garini, Alice Ya-Chun Chuang, Ian T Young,
Bart Vermolen, Richard van den Doe, Virginie Mougey, Mathilde Perrin, Martina Braun, Paul Donald Kerr,
Thierry Fest, Petra Boukamp and Sabine Mai1 (2004),
The three-dimensional organization of telomeres
in the nucleus of mammalian cells,
BMC Biology June 2004.
Santos, J.H., Meyer, J.N., Skorvaga, M., Annab, L.A., and Van Houten, B. (2004),
Mitochondrial hTERT exacerbases free-radical mediated mtDNA damage,
Aging Cell 3, 399-411.
[ Links/Mitochondrial hTERT,
Images,
Papers,
Patents,
Books].
Ralf Janknecht, edited by Varda Rotter (2004),
On
the road to immortality: hTERT upregulation in cancer cells,
FEBS Letters, 564, 1-2, 23 April 2004, pp.9-13.
JW Shay and
WE Wright (2005),
Senescence and Immortalization:
the role telomeres and telomerase,
Carcinogenesis,
Volume 26, Number 5 Pp. 867-874.
Jerry W. Shay and
Woodring E. Wright (2005),
Use of Telomerase to Create Bioengineered Tissues,
New York Academy of Sciences, 2005.
Maria A Blasco (2005),
Mice with bad ends:
mouse models for the study of telomeres and telomerase in cancer and aging,
The EMBO Journal,
(2005) 24, 1095 1103.
Judith Campisi (2005),
Senescent
Cells, Tumor Suppression, and Organismal Aging: Good Citizens, Bad Neighbor,
Cell Vol 120, Issue 4, 25 February 2005, Pages 513-522
Elizabeth H. Blackburn,
Carol W. Greider, and
Jack W. Szostack (2006),
(Nobel Prize in Medicine, 2009),
Telomeres
and Telomerase: the path from maize, Tetrahymena and yeast to human cancer and aging,
Nature Medicine,
vol. 12, no. 10, October 2006.
Maurelli, Zambruno, et al. (2006),
Inactivation of p16INK4a
(inhibitor of cyclin-dependent kinase 4A) Immortalizes Primary Human Keratinocytes By Maintaining Cells
in the Stem Cell Compartment,
The FASEB Journal, 2006;20:1516-1518.
Mogfield JE, Liu WR, Reid R (2006),
Adenoviral human
telomerase reverse transcriptase dramatically improves ischemic wound healing without detrimental
immune response in aged rabbit model,
Human Gene Therapy, 2006;
17(6): 651-660. This shows how telomerase
may be activated via gene therapy using a
viral vector.
Judy M.Y. Wong, and Kathleen Collins (2006),
Telomerase RNA level limits
telomere maintenance in X-linked dyskeratosis congenita,
Genes and Development
20:2848-2858, 2006.
Shay, J.W. and Wright, W.E. (2006),
Telomerase
therapeutics for cancer: challenges and new directions,
Nature Reviews Drug Discovery 5, 577-584.
Chun-Hong Zhu,
Vincent Mouly,
Racquel N. Cooper,
Kamel Mamchaoui,
Anne Bigot,
Jerry W. Shay,
James P. Di Santo,
Gillian S. Butler-Browne and
Woodring E. Wright (2007),
Cellular senescence in
human myoblasts is overcome by human telomerase reverse transcriptase and cyclin-dependent kinase 4:
consequences in aging muscle and therapeutic strategies for muscular dystrophies.,
Aging Cell 6(4): 515-523.
Christine Kim Garcia, Woodring E. Wright and
Jerry W. Shay (2007),
Human diseases of telomerase dysfunction:
insights into tissue aging,
Nucleic Acids Research,
2007, Vol. 35, No. 22 7406-7416.
Ramiro E. Verdun and
Jan Karlsreder (2007),
Replication
and protection of telomeres,
Nature Vol.447, 21 June 2007.
Qin Ouyang, Gabriela Baerlocher, Irma Vulto and Peter M. Lansdorp (2007),
Telomere Length in
Human Natural Killer Cell Subsets,
Hematopoietic Stem Cells
VI, Volume 1106 published June 2007,
Ann. N.Y. Acad. Sci.,
1106: 240 252 (2007).
Laure Crabbe, Anna Jauch, Colleen M. Naeger, Heidi Holtgreve-Grez, and Jan Karlseder (2007),
Telomere dysfunction as a cause of
genomic instability in Werner syndrome,
PNAS,
February 13, 2007, vol. 104, no. 7, 2205-2210.
H. Jiang, Z. Ju, and K. L. Rudolph (2007),
Telomere Shortening and Aging,
Zeitshrift fur Gerontol and Geriatrics,
40:314-324 (2007).
Wilson, James M, (2008),
Adverse
Events in Gene Transfer Trials and an Agenda for the New Year,
Human Gene Therapy,
Volume 19, #1, January, 2008.
Geraldine Aubert and Peter M. Lansdorp, (2008),
Telomeres and Aging,
Physiological Review
88: 557-579, 2008. Includes 248 footnotes.
Flores, I. et al. (2008),
The
longest telomeres: a general signature of adult stem cell compartments,
Genes and Development 22, 654-667.
Cong, Y. and Shay, J.W. (2008),
Actions
of human telomerase beyond telomeres,
Cell Research 18, 725-732.
Paul L, Cattaneo M, D'Angelo A, Sampietro F, Fermo I, Razzari C, Fontana G, Eugene N, Jacques PF, Selhub J. (2009),
Telomere
length in peripheral blood mononuclear cells is associated with folate status in men,
J Nutr. 2009 Jul;139(7):1273-8.
Tobias Else, (2009),
Telomeres and telomerase
in adrenocortical tissue maintenance, carcinogenesis, and aging,
Journal of
Molecular Endocrinology, (2009) 43, 131-141.
David R. Corey (2009),
Telomeres and Telomerase: From Discovery to Clinical Trials,
Chemistry & Biology, 16, Dec. 24, 2009.
Carroll, K.A., and Ly, H. (2009),
Telomere
dysfunction in human diseases: the long and short of it!,
J. Clin. Exp. Pathol. 2, 528-543.
Rochette, P.J. and Brash, D.E. (2010),
Human
Telomeres are hypersensitive to UV-induced DNA Damage and refractory to repair,
PLos Genet. 6, e1000926 (2010).
Murnane, J.P., (2010),
Telomere
loss as a mechanism for chromosome instability in human cancer,
Cancer Research 70, 4244-4259.
Ral, R. et. al. (2010),
The
function of classical and alternative non-homologous end-joining pathways in the fusion of dysfunctional telomeres,
EMBO Journal 29, 2598-2610.
Paula Martinez and Maria A. Blasco (2011),
Telomeric
and extra-telomeric roles for telomerase and the telomere-binding proteins,
Nature Reviews Cancer, vol. 11, March 2011, 161-176.
Are Telomeres the Key to Aging and Cancer?
from the
University of Utah's
Genetic Science Learning Center, with charts, photos, and illustrations
of telomere-related diseases.
Collected Papers of Elizabeth Blackburn:
[ Papers,
Patents,
Wikipedia,
Links].
Collected Papers of Carol W Greider
[ Papers,
Patents,
Wikipedia,
Links].
Collected Papers of Titia de Lange:
[ de Lange Lab,
de Lange Lab/Publications;
Papers,
Patents,
Wikipedia,
Links,
Books].
Collected Papers of C.B.Harley:
[ Papers,
Patents,
Links,
Abstracts].
Collected Papers of Eric Kool:
[ Papers,
Patents,
Links].
Collected Papers of Jerry W. Shay:
[ Papers,
Patents,
Links,
Shay/Wright Laboratory,
Resume w/Papers].
Collected Papers of Woodring E. Wright:
[ Papers,
Patents,
Links,
Resume w/Papers].
T.R. Cech, telomerase-related patents.
[ Patents,
Papers,
Links,
Books].
_____ 7.1 Aging Damage to Telomeres
_______[ Telomere Biology,
Replicative Senescence,
LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon,
Wikipedia/Telomere].
See also TA Sciences Science Library.
Judy M. Y. Wong,
Kathleen Collins (2003),
Telomere Maintenance and Disease,
The Lancet, May 13, 2003.
Bennet DC, Medrano EE, (2002),
Molecular Regulation
of Melanocyte Senescence,
Pigment Cell Research, 2002.
_____ 7.2 Small Molecule Telomerase Activators and related molecules
( (7)/Telomerase Activators,
Index,
List,
lifexrefs3.html)
____________ [ Index/Bioavailability,
LEF,
Links,
Images,
Papers,
Patents,
Books,
Amazon;
_____________ LEF,
Links/Activate Telomerase,
Images,
Papers,
Patents,
Books,
Amazon].
Cerni, C, (2000),
Telomeres, telomerase, and Myc,
Mutat. Res.,
Volume 462, Issue 1, January 2000, Pages 31-47.
Michael A. White, (2000),
METHODS AND
COMPOSITIONS FOR INHIBITING OR STIMULATING TELOMERASE ASSEMBLY,
____ Phosphoprotein 23 or
p23,
heat shock proteins
40,
70, and
90, and
HOP
(also called p60)
improve
telomerase assembly.
Helene LaBranche, Sophie Dupuis, Yaacov Ben-David, Maria-Rosa Bani, Raymund J. Wellinger, Benoit Chabot (1998),
Telomere
enlongation by hnRNP A1 and a derivative that interacts with telomeric repeats and telomerase,
Nature Genetics vol 19, June 1998.
The derivative was an exogenously applied amino acid fragment of hnRNP A1 named UP1 = UAGGGU.
UAGGGU (UP1) RNA can be applied exogenously to cell lysates to produce telomere elongation.
It corresponds to the DNA hex repeat TAGGGT, which can enlongate a chain of TTAGGG repeats when inserted
between the first two TTs of TTAGGG. TTAGGG -> T(TAGGGT)TAGGG = TTAGGGTTAGGG.
" UP1 promotes telomere enlongation in mammalian cells." Experiments are described
featuring myc-tagged UP1 inserted with a virus. UP1 recombinant retroviruses were used.
Kavita Y. Sarin,
Peggie Cheung,
Daniel Gilison,
Eunice Lee,
Ruth I. Tennen,
Estee Wang,
Maja K. Artandi,
Anthony E. Oro, and
Steven E. Artandi (2005),
Conditional telomerase induction
causes proliferation of hair follicle stem cells,
Nature, 2005 August 18; 436(7053): 1048-1052.
See also iGrowHair Telomerase,
which purports to
communicate
telomerase to the scalp using an acidophilus bacterium.
Christopher M. Counter
and Blaine N. Armbruster (2007),
Telomere Elongation,
United States Patent 20070020722, filed in 2004, published 2007.
Identifies a hPot1-hTERT fusion protein
deliverable in liposomes and otherwise
to rapidly enlongate telomeres.
____________ 7.2.1 Geron Telomerase Activators mostly based on Astragalus and Ginsenosides
[ 81s/6b, (7),
Refs3]
______________( 3b2/List,
US Patent 7,846,904 B2,
WO/2010/135247).
_____________[ LEF,
Links/Geron Telomerase Activators,
lifexrefs3.html,
Images,
Papers,
Patents,
Books,
Amazon;
_____________ Index/Astragalus,
Index/Astragalus Extract,
3b2,
LEF,
Links/Astragalus Extract,
Images,
Papers,
Patents,
Books,
Amazon;
_____________ Index/Ginseng,
3b2,
LEF,
Links/Ginsenosides,
Images,
Papers,
Patents,
Books,
Amazon;
_____________ Geron
A,
A',
A'',
B
| TA Sciences
| Sierra Sciences
| Maximum Telomere Support
| RevGenetics
| Terraternal].
Michael D. West,
Jerry Shay,
Woodring E. Wright (1997),
Methods and Reagents for Lengthening Telomeres,
United States Patent 5686306,
November 11, 1997, assigned to the Board of Regents,
The University of Texas System. Prescribes making available to the cell oligonucleotides
as nucleic acids typically including at least 2 to 3 telomeric repeat sequences,
which in humans is 5'-TTAGGG-3' (SEQ. ID. NO:2).
Michael D. West,
Jerry Shay,
Woodring E. Wright (1998),
Method for screening for agents
which increase telomerase activity in a cell, United States Patent 5830644,
assigned to Geron Corporation,
November 3, 1998. Contains many valuable references.
Hashimoto K., et.al, (2001 & 2003),
Skin
tissue regeneration promoters comprising ginsenoside Rb1,
United States Patent 20030104079.
Jean-Louis Mergny,
Jean-François Riou,
Patrick Mailliet,
Marie-Paule Teulade-Fichou and
Eric Gilson (2002),
Natural and pharmacological regulation of telomerase,
Nucleic Acids Research,
2002, Vol. 30, No. 4 839-865.
Lei Hong, Wang Ben, et al. (2003),
Anti-Aging Effects of Astragalosides
and its Mechanism of Action,
Acta Pharmacol Sin
March 2003 24 (3), 230-234.
____Mice: "The results of many studies showed that AST was
ineffective at 10, 20, 30 mg/kg, while it was effective at 40, 80, and 90 mg/kg."
____Long-telomere mice can tolerate large
astragalus extract doses until
antioxidant effects of
astragalus inhibit aging.
Lei H,
Wang B,
Li WP, Yang Y, Zhou AW, Chen MZ (2003),
Anti-aging
effect of astragalosides and its mechanism of action.,
Acta Pharmacol Sin.
2003 Mar;24(3):230-4.
Calvin B. Harley, et al. (2005),
Compositions
and Methods for Increasing Telomerase Activity,
( Geron patent ap.)
[ Papers,
Links].
___See also A' alternate version:
Compositions and Methods for
Increasing Telomerase Activity, or
A''.
See also US Patent 7,846,904 B2, Dec.7,2010..
Calvin B. Harley, Hong Kong U. Patent, (2005),
Formulations
Containing Astragalus Extracts and the Uses Thereof, European Patent Office.
Calvin, B. Harley, et. al, for
Geron Corporation, (2005),
Compositions and Methods
for Skin Conditioning
including small molecule telomerase activators
such as astragaloside IV,
cycloastragenol,
astragenol, and
astragaloside IV 16-one in glycerin, ect.
Calvin B Harley (2005),
Telomerase Therapeutics for Degenerative Diseases,
Current Molecular Medicine,
Volume 5, Number 2, March 2005 , pp. 205-211(7).
Steven Russell Fauce,
Beth D. Jamieson,
Allison C. Chin,
Ronald T. Mitsuyasu,
Stan T. Parish,
Hwee L. Ng,
Christina M. Ramirez Kitchen,
Otto O. Yang,
Calvin B. Harley and
Rita B. Effros (2008),
Telomerase-Based Pharmacologic Enhancement
of Antiviral Function of Human CD8+ T Lymphocytes,
The Journal of Immunology, 2008,
181: 7400-7406. Test of telomerase activator
TAT2
( cycloastragenol).
"Enhanced antiviral effects were abrogated in the presence of a potent and specific
telomerase inhibitor,
suggesting that TAT2 acts primarily through telomerase activation."
See also the associated Geron News Release.
Min Yanga, Xin-Hua Qian, Dan-Hua Zhaoa and Su-Zhen Fu (2009).
Effects
of Astragalus polysaccharide on the erythroid lineage and microarray analysis in K562 cells,
Journal of Ethnopharmacology,
Nov 2009.
Geron (2010) intellectual property
WO/2010/135247
( direct) for
Enhanced-bioavailability
telomerase activators.
________ 7.2.2 Bioavailablility of Astragalosides and Small-Molecule Telomerase Activators
_____________[ Index/Bioavailability,
LEF,
Links/Bioavailability,
Images,
Papers,
Patents,
Books,
Amazon;
_____________ Links/Bioavailability of Astragalosides,
Images,
Papers,
Patents,
Books,
Amazon;
Chitosan,
Sodium Deoxycholate].
Takakura, et al. (2001),
Telomerase activation by
histone deacetylase inhibitor in normal cells,
Nucleic Acids Research,
2001, Vol. 29, No. 14 3006-3011
Jean-Louis Mergny, et al., (2002),
Natural and Pharmacological Regulation of Telomerase,
Nucleic Acids Research,
2002, volume 30, number 4, 839-864.
Maria Jose Cano-Cebrian,
et al., (2005),
Intestinal Absorption Enhancement
via the Paracellular Route by Fatty Acids, Chitosans, and Others: A Target for Drug Delivery,
Current Drug Delivery,
2005, 2, 9-22. Chitosan increases the bioavailability of astragaloside IV.
Huang CR, Wang GJ, Wu XL, Li H, Xie HT, Lv H, Sun JG, (2006),
Absorption
enhancement study of astragaloside IV based on its transport mechanism in caco-2 cells,
Eur J
Drug Metab Pharmacokinet. 2006 Jan-Mar;31(1):5-10.
Huang CR, Wang GJ, Wu XL, Li H, Xie HT, Lv H, Sun JG, (2006),
Absorption
enhancement study of astragaloside IV based on its transport mechanism in caco-2 cells,
Eur J
Drug Metab Pharmacokinet,
2006, Jan-Mar;31(1):5-10.
The bioavailability of
astragaloside IV
[ Article,
Papers],
can be increased by
chitosan
[ Wiki/chitosan,
Links/chitosan]
or sodium
deoxycholate
[ Links/sodium deoxycholate,
Books].
Calvin B Harley,
Xu-Rong Jiang,
Choy-Pik Chiu (2006),
Enhancing cell migration by increasing
telomerase activity,
United States
Patent 20060239974.
Arlona Tarkanyi, PhD thesis,
Pharmacological
Regulation of Telomerase:..., phd.okm.gov.hu.
Won, J., Kim, M., Kim, N., Ahn, J.H., Lee, W.G., Kim, S.S., Chang,K.-Y., Yi, Y.-W., and Kim, T.K. (2006),
Small
molecule-based reversible reprogramming of cellular lifespan,
Nat. Chem. Bio. 2:369-74, 2006.
Geron (2010) intellectual property
WO/2010/135247
( direct) for
Enhanced-bioavailability
telomerase activators.
______ 7.2.3 DNA Nanocircles and Telomere Elongation: Eric T. Kool's Work.
[ Amazon].
_____________[ LEF,
Links/DNA Nanocircles for Telomere Enlongation,
Images,
Papers,
Patents,
Books,
Amazon;
_____________ Links/Eric T Kool Telomere Enlongation,
Images,
Papers,
Patents,
Books,
Amazon].
Ulf M. Lindstrom,
Ravi A. Chandrasekaran,
Lucian Orbai,
Sandra A. Helquist,
Gregory P. Miller,
Emin Oroudjev,
Helen G. Hansma, and
Eric T. Kool (2002),
Artificial human telomeres
from DNA nanocircle templates,
PNAS,
December 10, 2002, vol. 99, no. 25, 15953-15958.
Dr. Eric T. Kool, (2003),
(WO/2003/057849)
TELOMERE-ENCODING SYNTHETIC DNA NANOCIRCLES, AND THEIR USE FOR THE ELONGATION OF TELOMERE REPEATS,
World Intellectual Property Organization.
Jorg S. Hartig and
Eric T. Kool (2004),
Small circular DNAs
for synthesis of the human telomere repeat: varied sizes, structures and telomere-encoding activities,
Nucleic Acids Research
2004 vol.32(no.19): pg.152.
______ Temporary Catch-All
Alan K. Meeker,
Jessica L. Hicks,
Christine A. Iacobuzio-Donahue,
Elizabeth A. Montgomery,
William H. Westra,
Theresa Y. Chan,
Brigitte M. Ronnett and
Angelo M. De Marzo, (2004),
Telomere Length Abnormalities
Occur Early in the Initiation of Epithelial Carcinogenesis,
Clinical Cancer Research
Vol. 10, 3317-3326, May 15, 2004.
Bekaert s, Koll S, et al. (2002),
Comparing
telomere lengths of sister chromatids in human lymphocytes
using 3-dimensional confocal microscopy,
Cytometry, 2002.
Yong-Ouk You,
Gene Lee and
Byung-Moo Min, (2000),
Retinoic Acid
Extends the in Vitro Life Span of Normal Human Oral Keratinocytes by Decreasing p16INK4A Expression
and Maintaining Telomerase Activity,
Biochemical
and Biophysical Research Communications,
Volume 268, Issue 2, 16 February 2000, Pages 268-274.
Maurelli, Zambruno, et al. (2006),
Inactivation of p16INK4a
(inhibitor of cyclin-dependent kinase 4A) Immortalizes Primary Human Keratinocytes By Maintaining Cells
in the Stem Cell Compartment,
The FASEB Journal, 2006;20:1516-1518.
Kayo Furumotoa,
Eiji Inouea,
Norio Nagaoa,
Eiso Hiyamab and
Nobuhiko Miwa (1998),
Age-dependent
telomere shortening is slowed down by enrichment of intracellular
vitamin C via suppression of oxidative stress,
Life Sciences,
Volume 63, Issue 11, 7 August 1998, Pages 935-948.
HANNON, Gregory,
Jing J. WANG,
BEACH, David, H. (1999),
(WO/1999/035243)
EXTENSION OF CELLULAR LIFESPAN, METHODS AND REAGENTS,
[ Links].
Hideki Mizuno,
Shilagardi Khurts,
Takahiko Seki,
Yasuhide Hirota,
Shuichi Kaneko and
Seishi Murakami (2007),
Human Telomerase Exists in
Two Distinct Active Complexes In Vivo (one is complexed with
HSP 90),
Journal of Biochemistry,
2007 141(5):641-652.
Richard C. Allsopp,
Samuel Cheshier and
Irving L. Weissman (2002),
Telomerase Activation and Rejuvenation
of Telomere Length in Stimulated T Cells Derived from Serially Transplanted Hematopoietic Stem Cells,
JEM, 2 December 2002.
Antigenic
stimulation activates telomerase in T-cells.
_____ 7.2.4 Sierra Sciences: the hTERT Promoter and Methods and Compositions for modulating hTERT expression
[ Sierra Sciences].
_____________[ LEF/William Andrews,
Sierra Sciences,
Links/Sierra Sciences,
Images,
Papers,
Patents,
Books;
_____________ Links/the hTERT promoter,
Images,
Papers,
Patents,
Books,
Amazon;
_____________ LEF/modulating hTERT expression,
Links,
Images,
Papers,
Patents,
Books,
Amazon].
( WO/2002/101010)
______________ METHODS
AND COMPOSITIONS FOR MODULATING
______________TELOMERASE REVERSE TRANSCRIPTASE (TERT) EXPRESSION].
William H. Andrews,
C.A.Foster,
S. Fraser,
Hamid Mohammedpour, of
Sierra Sciences (2004):
Methods
and Compositions for Modulating Telomerase Reverse Transcriptase (TERT) Expression,
United States Patent No. 6,686,159 B2,
Andrews, et al., Feb 3, 2004. Explains the hTERT repressor
in the hTERT promotor and means of interfering with
hTERT repression.
William H. Andrews,
Laura Briggs,
Lancer K. Brown,
Christopher A. Foster,
Mieczyslaw A. Piatyszek,
Assignee:
Sierra Sciences, Inc. (2005),
Assays for TERT promoter modulatory agents...,
US Patent # 7226744.
Andrews; William H.,
Foster; Christopher A.,
Fraser; Stephanie,
Mohammadpour; Hamid,
Briggs; Laura,
Assignee: Sierra Sciences, Inc. (2004),
Methods
and compositions for modulating telomerase reverse transcriptase (TERT) expression.,
Appl. No.: 10/826,466.
Yi-hsin HSU,
Jing-jer LIN
(2005),
Telomere
and telomerase as targets for anti-cancer and regeneration therapies,
Acta Pharmacologica Sinica,
2005 May; 26 (5): 513-518.
___________ 7.2.5 Nitric Oxide as a Telomerase Activator
______________[ Index/Nitric Oxide,
LEF/Nitric Oxide,
NO More Heart Disease,
______________ Links/Nitric Oxide activates telomerase,
Images,
Papers,
Patents,
Books].
______________[ LEF,
Links/Nitric Oxide as a Telomerase Activator,
Images,
Papers,
Patents,
Books].
M. Vasa, K Breitschopf, AM Zeiher, S Dimmeler (2000),
Nitric Oxide
Activates Telomerase and Delays Endothelial Cell Senescence,
Circulation Research, 2000, 87: pp. 450-452. - Am Heart Assoc.
____PDF sup_notes: Nitric Oxide
Activates Telomerase and Delays Endothelial Cell Senescence, Univ. of Frankfort.
Toshio Hayashi, Hisako Matsui-Hirai, Asaka Miyazaki-Akita, Akiko Fukatsu, Jun Funami,
Qun-Fang Ding, Sumitra Kamalanathan, Yuichi Hattori, Louis J. Ignarro, and Akihisa Iguchi (2006),
Endothelial cellular senescence
is inhibited by nitric oxide: Implications in atherosclerosis associated with menopause and diabetes,
PNAS,
November 7, 2006, vol. 103, no. 45, pp. 17018-17023.
Erusalimsky JD, (2009),
Vascular endothelial senescence:
from mechanisms to pathophysiology,
Journal of
Applied Physiology, 106/1/326, 2009.
Hong Y, Quintero M, Frakich NM, Trivier E, Erusalimsky JD (2007),
Evidence against the involvement of nitric oxide in the modulation of telomerase activity
or replicative capacity of human endothelial cells,
Experimental Gerontology
42: 904-910, 2007.
Judith Haendeler (2006),
Nitric oxide
and endothelial cell aging,
European Journal of
Clinical Pharmacology, Volume 62, Supplement 1 / February, 2006.
Farsetti A, Grasselli A, Bacchetti S, Gaetano C, Capogrossi MC (2009),
The telomerase tale in vascular aging:
regulation by estrogens and nitric oxide signaling,
Journal of Applied Physiology
106: 333-337, 2009.
___________ 7.2.6 Novel Wistar Institute Small-Molecule Telomerase Activators
____________[ Tri-Phenyl Compounds,
Links/Wistar Institute Telomerase Activators,
Images,
Papers,
Patents,
Books].
See Aviv Gazit et al (2008),
TELOMERASE
ACTIVATING COMPOUNDS AND METHODS OF USE THEREOF,
US Patent,
US Patent App. 12/602,956, 2008,
WO Patent WO/2008/149,353, 2008. (See PDF version.)
Tri-Phenyl
Compound Telomerase Activators
developed at Ben Gurion
University on the Negev
[ Links,
Images,
Papers,
Books].
Novel
Telomerase Activators,
Ben Gurion University
on the Negev. BGN Technologies.
Andrew J. Gillis, Anthony, P. Schuller &
Emmanuel Skordalakes (2008),
Structure
of the Tribolium castaneum telomerase catalytic subunit TERT,
Nature, 455:633-637, 2008.
Referenced in The Wistar Institute/Design
of Novel Molecules That Regulate Telomerase.
Rouda S, Skordales E. Structure, (2007), Nov. 15(11):1403-12,
Structure
of the RNA binding domain of telomerase: Implications for RNA recognition and binding,
Referenced in The Wistar Institute/Design
of Novel Molecules That Regulate Telomerase.
____See Decipering Of
Telomerase Structure Opens Door To New Cancer, Aging Treatments at
Medical News Today
describes work by Emmanuel Skordalakes
on telomerase structure.
___________ 7.2.7 Androgens as Small-Molecule Telomerase Activators
___________[ List/Androgens,
Links,
Images,
Papers,
Patents;
Books/Androgens and Aging;
LEF/Androgens,
Wikipedia].
Rodrigo T. Calado, William T. Yewdell, Keisha L. Wilkerson, Joshua A. Regal,
Sachiko Kajigaya, Constantine A. Stratakis, and Neal S. Young (2009),
Sex hormones,
acting on the TERT gene, increase telomerase activity in human primary hematopoietic cells,
Blood,
10 September 2009, Vol. 114, No. 11, pp. 2236-2243.
Chuanhai Guo, Blaine N. Armbruster, David T. Price, Christpher M. Counter, (2003),
In Vivo Regulation of
hTERT Expression and Telomerase Activity by Androgen,
Journal of the
American Academy of Dermatology, Volume 170, Issue 2, Part 1,
Pages 615-618 (August 2003).
____Other investigators speculate that androgens are converted to
estrogens
before interacting with
the hTERT promoter to activate telomerase.
_____ 7.3 Telomerase Inhibitors
[ SupNotes3b/telomerase inhibitors,
List,
LEF,
Links/Telomerase Inhibitors,
Images,
Papers,
Patents,
Books].
Mayank Singh and Neeta Singh, (2009),
Molecular mechanism of
curcumin induced cytotoxicity in human cervical carcinoma cells,
Molecular and Cellular Biochemistry,
Volume 325, Numbers 1-2 / May, 2009, Pages 107-119.
Theeffectiveness of curcumin for this application
derives from its properties as a
telomerase inhibitor.
Park SE, Park C, Kim SH, Hossain MA, Kim MY, Chung HY, Son WS, Kim GY, Choi YH, Kim ND (2009),
Korean red ginseng extract induces apoptosis and decreases
telomerase activity in human leukemia cells.,
Journal of Ethnopharmacology,
2009 Jan 21;121(2):304-12.
Moriai M, Tsuji N, Kobayashi D, Kuribayashi K, Watanabe N, (2009),
Down-regulation of hTERT expression plays an important role in
15-deoxy-Delta12,14-prostaglandin J2-induced apoptosis in cancer cells,
1: International Journal of Oncology,
2009 May; 34(5): 1363-1372.
____ 7.4 The hTERT Promoter
[ Index,
Links/hTERT Promoter,
Images,
Papers,
Patents,
Books].
Horikawa I., Cable P. L., Afshari C., Barrett J. C. (1999),
Cloning
and characterization of the promoter region of human telomerase reverse transcriptase gene,
Cancer Research, 59: 826-830, 1999.
William H. Andrews, C.A.Foster, S. Fraser, Hamid Mohammedpour, of
Sierra Sciences (2004):
Methods
and Compositions for Modulating Telomerase Reverse Transcriptase (TERT) Expression,
United States
Patent No. 6,686,159 B2, Andrews, et al., Feb 3, 2004. Explains the hTERT repressor
in the hTERT promotor
and means of interfering with hTERT repression.
William H. Andrews, Laura Briggs, Lancer K. Brown, Christopher A. Foster, Mieczyslaw A. Piatyszek, Assignee:
Sierra Sciences, Inc. (2005),
Assays for TERT promoter modulatory agents...,
Telomerase promoter driving expression of therapeutic gene sequences.
M Wick, D Zubov, G Hagen (1999),
Genomic organization and promoter characterization of the gene
encoding the human telomerase reverse transcriptase (hTERT),
Gene,
Volume 232, Issue 1, 17 May 1999, Pages 97-106.
Cong, YS (1999),
The Human Telomerase Catalytic Subunit hTERT:
organization of the gene and characterization of the promoter,
Human Molecular Genetics, 1999, vol.8, no.1,
137-142.
Yu-Sheng Cong, Woodring E. Wright, and
Jerry W. Shay,
Human Telomerase and Its Regulation.
Izumi Horikawa, P. LouAnn Cable, Cynthia Afshari and J. Carl Barrett, (1999),
Cloning and Characterization of the
Promoter Region of Human Telomerase Reverse Transcriptase Gene ,
Cancer Research, 59, 826-830,
February 1, 1999.
[ Papers,
Books].
Takakura, M., Kanaya,T., et al (1999),
Cloning of human telomerase
catalytic subunit (hTERT) gene promoter and identification of proximal core
promoter sequences essential for transcriptional activation in immortalized
and cancer cells,
Cancer Research, 59, 551-557.
M.P. Mattson (2001),
Telomerase,
Aging and Disease (Advances in Cell Aging and Gerontology),
Elsevier, 2001.
Wu, (1999),
c-Myc activates hTERT promoter,
Nature Genetics, 1999.
[ Links,
Papers,
Books].
Theodora R. Devereux, Izumi Horikawa, Colleen H. Anna, Lois A. Annab, Cynthia A. Afshari and J. Carl Barrett, (1999),
DNA Methylation Analysis of the Promoter Region
of the Human Telomerase Reverse Transcriptase (hTERT) Gene,
Cancer Research
59, 6087-6090, December 1, 1999.
Joseph C. Poole, Lucy G. Andrews and Trygve O. Tollefsbol (2001),
Activity,
function, and gene regulation of the catalytic subunit of telomerase (hTERT),
Gene,
Volume 269, Issues 1-2, 16 May 2001, Pages 1-12.
Sang Sun Kang, Taegun Kwon, Do Yoon Kwon, and Su Il Do, (1999),
Akt Protein Kinase Enhances Human Telomerase
Activity through Phosphorylation of Telomerase Reverse Transcriptase Subunit,
Journal of Biological Chemistry,
Vol. 274, Issue 19, 13085-13090, May 7, 1999.
_____ 7.5 Telomere Epigenetics
[ Links/Epigenetics;
Links/Telomere Epigenetics,
Images,
Papers,
Patents,
Books;
Books/Epigenetic Control;
_______ Index/Telomeric Chromatin].
Theodora R. Devereux, Izumi Horikawa, Colleen H. Anna, Lois A. Annab, Cynthia A. Afshari and J. Carl Barrett (1999),
DNA Methylation Analysis of the Promoter
Region of the Human Telomerase Reverse Transcriptase (hTERT) Gene,
Cancer Research 59, 6087-6090,
December 1, 1999.
This paper includes an analysis of
the hTERT promoter.
" Most of the hTERT-negative normal cells and about one-third of the hTERT-expressing cell lines
had the unmethylated/hypomethylated promoter, whereas the other hTERT-expressing cell lines
showed partial or total methylation of the promoter. The promoter of one hTERT-negative
fibroblast cell line, SUSM-1, was methylated at all sites examined."
Farwell, Shera, et al. (2000),
Genetic and Epigenetic Changes
in Human Epithelial Cells Immortalized by Telomerase,
American Journal of Pathology
2000 May; 156(5): 1537 1547.
___It may be necessary to down-regulate p16INK4a and
p14ARF in breast cell or foreskin tissue for
these to become immortal.
Kiyoaki Nomoto1, Masato Maekawa1, Kokichi Sugano1, Mineko Ushiama1,
Noriko Fukayama1, Shin Fujita2 and Tadao Kakizoe3 (2002),
Methylation
Status and Expression of Human Telomerase Reverse Transcriptase mRNA in Relation to Hypermethylation
of the p16 gene in Colorectal Cancers as Analyzed by Bisulfite PCR-SSCP,
Jpn. J. Clin. Oncol. (2002) 32 (1): 3-8.
Isabelle Guilleret,
Pu Yan (2002),
Hypermethylation
of the human telomerase catalytic subunit (hTERT) gene correlates with telomerase activity,
International Journal of Cancer,
Volume 101, Issue 4 , Pages 335 - 341, 7 Aug 2002.
Widschwendter A, Müller HM, Hubalek MM, Wiedemair A, Fiegl H, Goebel G,
Mueller-Holzner E, Marth C, Widschwendter M. (2004),
Methylation
status and expression of human telomerase reverse transcriptase in ovarian and cervical cancer,
Gynecol Oncol. 2004 May;93(2):407-16.
" No correlation was detected between
hypermethylation of hTERT and hTERT mRNA expression.".
Maria A. Blasco (2004),
Telomere epigenetics:
a higher-order control of telomere length in mammalian cells,
Carcinogenesis,
Vol. 25, No. 7, 1083-1087, July 2004.
___ hRAP1 is
recruited by
TRF2 to human telomeres.
hRAP1 is the homologue of yeast RAP1 protein and its
over-expression
causes telomere elongation.
Garcia-Cao, M., O'Sullivan, R., Peters, A.H., Jenuwein, T., and Blasco, M.A. (2004),
Epigenetic
regulation of telomere length in mammalian cells by SUV39h1 and SUV39h2 histone methyltransferases,
Nature Genetics 36, 94-99.
Gonzalo, S., et al. (2005),
The
role of the RB1 family in stabilizing histone methylation at constitutive heterochromatin,
Nature Cell Biology 7, 420-428.
Gonzalo, S. et al. (2006),
DNA
methylases control telomere length and telomere recombination in mammalian cells,
Nature Cell Biology 8, 416-424.
Rebekah L. Zinn, Kevin Pruitt, Sayaka Eguchi, Stephen B. Baylin, and James G. Herman, (2007),
hTERT
is Expressed in Cancer Cell Lines Despite Promoter DNA Methylation by
Preservation of Unmethylated DNA and Active Chromatin around the Transcription Start Site,
Cancer Research 2007 67; (1) January 1, 2007, pp.194-201.
Azzalin, C.M., Reichenbach, P. Khorlauli, L., Glulotto, E., and Lingner, J. (2007),
Telomeric
repeat containing RNA and RNA surveillance factors at mammalian chromosome ends,
Science 318, 789-801.
Benetti, R. et al. (2007),
SUV4-20h
deficiency results in telomere enlongation and derepression of telomere recombination,
Journal of Cell Biology 178, 925-936.
Benetti, R., Garcia-Cao,M. and Blasco, M.A. (2007),
Telomere
length regulates the epigenetic status of mammalian telomeres and subtelomeres,
Nature Genetics 39, 243-250.
Shoeftner, S., and Blasco, M.A. (2008),
Developmentally
regulated transcription of mammalian telomeres by DNA-dependent RNA polymerase II,
Nature Cell Biol. 10, 228-236.
Benetti, R., et al. (2008),
A
mammalian microRNA cluster controls DNA methylation and telomere recombination via Rb12-dependent regulation
of DNA methyltransferases,
Nature Struct. Mol. Biol 15, 268-279.
See Schoeftner, S. and Blasco, M.A. (2009),
A
'higher order' of telomere regulation: telomere heterochromatin and telomeric RNAs,
EMBO Journal 28, 2323-2336.
Marion, R.M., et al. (2009),
Telomeres
acquire embryonic stem cell characteristics in induced pluripotent stem cells,
Cell Stem Cell 4, 141-154.
Describes epigenetic markers of Embyronic Stem cells during nuclear reprogramming.
Paula Martinez and Maria A. Blasco (2011),
Telomeric
and extra-telomeric roles for telomerase and the telomere-binding proteins,
Nature Reveiws Cancer, vol. 11, March 2011, 161-176.
_____ 7.6 The Telomere Position Effect and Youthful Patterns of Gene Expression
_______[ Links/The Telomere Position Effect,
Images,
Video,
Papers,
Books].
DE Gottschling, OM Aparicio, BL Billington, VA, et al. (1990),
Position
effect at S. cerevisiae telomeres: reversible repression of Pol II transcription,
Cell, Vol 63, 751-762, 16 November 1990.
Wright, W. E. and J.W.Shay (1992),
Telomere
positional effects and the regulation of cellular senescence,
Trends Genet 8:193-197.
Joseph A. Baur, Ying Zou,
Jerry W. Shay,
Woodring E. Wright (2001),
Telomere Position Effect in Human Cells,
Science
15 June 2001: Vol. 292. no. 5524, pp. 2075 - 2077.
Carmia Borek (2002),
Telomere
Control and Cellular Aging,
Life Extension Magazine, September 2002.
Joseph A. Baur, Woodring E. Wright,
Jerry W. Shay (2004),
Analysis
of Mammalian Telomere Position Effect,
Epigenetics Protocols, 2004.
______ 7.7 Telomere T-Loop and D-Loop
_________[ Links/The Telomere t-loop,
Images,
Video,
Papers,
Books;
Links/The Telomere d-loop,
Images,
Video,
Papers,
Books].
Carol W. Greider (1999),
Telomeres
Do D-Loop T-Loop Minireview.
Jerry W. Shay (1999),
On telomeric t-loops and d-loops.
J.L. Munoz-Jordan, G.A. Cross, T. de Lange, and J. D. Griffith (2001),
T-loops at typanosome telomeres,
EMBO Journal 20, 579-588.
_____ 7.8 Telomere Capping Proteins
________[ Index/Telomere Loop Control Proteins,
Index/Mammalian Telomere Protein Factors
with The Telomere Interactome,
________ Links/Telomere Capping Proteins,
Images,
Video,
Papers,
Books;
Amazon/Shelterin,
Amazon/The Telosome].
Lustig, A.J., Kurtz, S., and Shore, D. (1990),
Involvement
of the silencer and UAS binding protein RAP1 in regulation of telomere length,
Science 250, 549-553.
Fang G, Cech TR (1993),
The
beta subunit of Oxytricha telomere-binding protein promotes G-quartet formation by telomeric DNA,
Cell 1993, 74:875-885.
[ Links/DNA G-quartet formation].
Henrik Tommerup, Athanasios Dousmanis, and Titia de Lange (1994),
Unusual
Chromatin in Human Telomeres,
Molecular and Cellular Biology, Sept. 1994, p.5777-5785.
Chong, L., van Steensel, B., Broccoli, D., Erdjument-Bromage, H., Hannish, J., Tempst, T., and de Lange, T., (1995),
A human telomeric protein
( TRF1),
Science 270, 1663-1667.
A. Krauskopf, Elizabeth H. Blackburn (1996),
Control
of telomere growth by interactions of RAP1 with the most distal telomeric repeats,
Nature 383, 354-357.
[ Shelterin/RAP1].
Nugent, C. I., T. R. Hughes, N. F. Lue, and V. Lundblad (1996),
Cdc13p:
a single-strand telomeric DNA-binding protein with a dual role in yeast telomere maintenance,
Science 274:249-252. [ Links/Cdc13p].
Broccoli D, Smogorzewska A, Chong L, de Lange T (1997),
Human
telomeres contain two distinct Myb-related proteins, TRF1 and TRF2,
Nature Genetics 1997, 17:231-235.
[ Links/Myb-related proteins,
Shelterin/TRF1,
Shelterin/TRF2].
Bilaud T, Brun C, Ancelin K, Koering CE, Laroche T, Gilson E (1997),
Telomeric
localization of TRF2, a novel human telobox protein,
Nature Genetics 1997, 17:236-239.
[ Links/telobox proteins,
Shelterin/TRF2].
Bianchi, A, Smith, S, Chong, L, Elias, P, and de Lange, T. (1997),
TRF1
is a dimer and bends telomeric DNA,
EMBO Journal, 16: 1785-1784.
[ Shelterin/TRF1].
Shen M, Haggblom C, Vogt M, Hunter T, Lu KP (1997):
Characterization
and cell cycle regulation of the related human telomeric proteins Pin2 and TRF1 suggest a role in mitosis,
Proc Natl Acad Sci USA 1997, 94:13618-13623.
[ Shelterin/TRF1,
Pin2].
van Steensel B, de Lange T (1997),
Control
of telomere length by the human telomeric protein TRF1,
Nature 1997, 385:740-743.
[ Shelterin/TRF1].
Bryan TM, Englezou A, Dalla-Pozza L, Dunham MA, Reddel RR (1997),
Evidence
for an alternative mechanism for maintaining telomere length in human tumors and tumor-derived cell lines,
Nature Med 1997, 3:1271-1274.
Horvath MP, Schweiker VL, Bevilacqua JM, Ruggles JA, Schultz SC (1998),
Crystal
structure of the Oxytricha nova telomere end binding protein complexed with single strand DNA,
Cell 1998, 95:963-974.
Smith S, Giriat I, Schmitt A, de Lange T (1998),
Tankyrase,
a poly(ADP-ribose) polymerase at human telomeres,
Science 1998, 282:1484-1487.
[ List(Telomerase Activators)/Tankyrase].
van Steensel B, Smogorzewska A, de Lange T (1998),
TRF2
protects human telomeres from end-to-end fusions,
Cell 1998, 92:401-413.
[ Shelterin/TRF2].
d'Adda di Fagagna, F., Hande, M.P., Tong, W.-M., Lansdorp, P.M., Wang, Z.Q., Jackson, S.P. (1999),
Functions
of poly(ADP-ribose) polymerase in controlling telomere length and chromosomal stability,
Nature Genetics 23, 76-80.
Karlsreder J., Broccoli, D., Dai, Y., Hardy, S., and de Lange, T. (1999),
P53
and ATM-dependent apoptosis induced by telomeres lacking TRF2,
Science 283, 1321-1325. [ Shelterin/TRF2].
S. Le, J.K. Moore, J.E. Haber, and Carol W. Greider (1999),
RAD50
and RAD51 define two pathways that collaborate to maintain telomeres in the absence of telomerase,
Genetics 152, 143-152.
[ Telomere Management Proteins/RAD51D,
Telomere Interactome/RAD50].
S. H. Kim, P. Kaminker, and Judith Campisi (1999),
TIN2,
a new regulator of telomere length in human cells,
Nature Genetics 23, 405-412.
[ Shelterin/TIN2].
K.G. Murti and D.M.Prescott (1999),
Telomeres
of polytene chromosomes in a ciliated protozoan terminate in duplex DNA loops,
PNAS,
December 7, 1999, Vol 96, No. 25, pp.14436-14439.
Griffith JD, Comeau L, Rosenfield S, Stansel RM, Bianchi A, Moss H, de Lange T (1999),
Mammalian
telomeres end in a large duplex loop,
Cell 1999, 97:503-514.
Karlseder J, Broccoli D, Dai Y, Hardy S, de Lange T (1999),
p53-
and ATM-dependent apoptosis induced by telomeres lacking TRF2,
Science 1999, 283:1321-1325.
[ Shelterin/TRF2,
Telomere Interactome/ATM,
p53].
Iftode C, Daniely Y, Borowiec JA (1999),
Replication
protein A (RPA): the eukaryotic SSB,
Crit Rev Biochem Mol Biol 1999, 34:141-180.
Smith S, de Lange T (1999),
Cell
cycle dependent localization of the telomeric PARP, tankyrase, to nuclear pore complexes and centrosomes,
Journal of Cell Science 1999, 112:3649-3656.
Huffman, K.E., Levene, S.D., Tesmer, V.M., Shay, J.W., Wright, W.E. (2000),
Telomere
shortening is proportional to the size of the G-rich telomeric 3'-overhang,
Journal of Biological Chemistry, 275, 19719-19722.
Song K, Jung D, Jung Y, Lee S.G, Lee I (2000),
Interaction
of human Ku70 with TRF2,
FEBS Letters 2000, 481:81-85.
[ Shelterin/TRF2,
Ku70].
Zhu XD, Kuster B, Mann M, Petrini JH, de Lange T (2000),
Cell-cycle-regulated
association of RAD50/MRE11/NBS1 with TRF2 and human telomeres,
Nature Genetics, 2000, 25:347-352.
[ Shelterin/TRF2,
RAD50,
MRE11,
NBS1].
Smith S, de Lange T (2000),
Tankyrase
promotes telomere elongation in human cells,
Curr Biol 2000, 10:1299-1302. [ TA/Tankyrase].
Nai-Wen Chi and Harvey F. Lodish (2000),
Tankyrase
Is a Golgi-associated Mitogen-activated Protein Kinase Substrate That Interacts with IRAP in GLUT4 Vesicles,
Journal of Biological Chemistry, December 8, 2000, 275, 38437-38444. This paper describes the phosphorylation of
tankyrase 1 in insulin-stimulated cells.
" Tankyrase is a novel
signaling target of
mitogen-activated protein kinase
(MAPK);
it is stoichiometrically
phosphorylated
upon insulin stimulation
[Images,
Papers,
Patents,
Books].
Phosphorylation enhances
the
poly(ADP-ribose) polymerase activity of tankyrase... (requiring a
NAD+
( Nicotinamide adenine dinucleotide) substrate)...
The phosphorylation of tankyrase by MAPK increases its PARP activity..." Chi and Lodish
also found tankyrase 1 phosphorylation due to stimulation of tankyrase 1 with
PDGF and EGF.
" ...Tankyrase is quantitatively phosphorylated on
certain serine residues by MAPK upon stimulation with insulin, PDGF, and EGF."
Phosphorylation allows tankyrase to transduce MAPK signaling into poly(ADP-ribosyl)ation of effector proteins.
H.L Hsu, , D. Gilley, S.A. Galande, M.P. Hande, B. Allen, S.H. Kim, S.H., G.C. Li, Judith Campisi,
T. Kohwi-Shigematsu, and D.J. Chen, (2000).
Ku
acts in a unique way at the mammalian telomere to prevent end joining,
Genes and Development, 14, 2807-2812.
The Ku proteins
are often represented by 2011 as binding at spot where the 3' strand separates from duplex telomere DNA.
Li B, Oestreich S, de Lange T (2000),
Identification
of human Rap1: implications for telomere evolution,
Cell 2000, 101:471-483.
Smogorzewska A, van Steensel B, Bianchi A, Oelmann S, Schaefer MR, Schnapp G, de Lange T (2000),
Control
of human telomere length by TRF1 and TRF2,
Mol Cell Biol 2000, 20:1659-1668.
Zhou BB, Elledge SJ (2000),
The
DNA damage response: putting checkpoints in perspective, .
Nature 2000, 408:433-439.
Li, B, and Comai, L. (2000),
Functional
interaction between KU and the werner syndrome protein in DNA end processing,
Journal of Biological Chemistry 275: 28349-28352.
Ghosh, I., Hamilton, A.D., and Regan, L. (2000),
Antiparallel
Leucine Zipper-Directed Protein Reassembly: Application of Green Fluorescent Protein,
Chem Soc 122, 5658.
Blackburn, E. H. (2000),
Telomere states and cell fates,
Nature 408:53-56.
Chandra, A., T. R. Hughes, C. I. Nugent, and V. Lundblad (2001),
Cdc13
both positively and negatively regulates telomere replication,
Genes and Development, 15:404-414.
Kishi S, Zhou XZ, Ziv Y, Khoo C, Hill DE, Shiloh Y, Lu KP (2001),
Telomeric
protein Pin2/TRF1 as an important ATM target in response to double strand DNA breaks,
J Biol Chem 2001, 276:29282-29291.
S. E. Peterson, A.E. Stellwagen, S.J. Diede, M.S. Singer, Z.W. Haimberger, C.O. Johnson, M. Tzoneva, and
D.E. Gottschling (2001),
The function of a stem-loop in telomerase RNA is linked to the DNA repair protein Ku,
Nature Genetics 27, 64-67.
Baumann P, Cech TR (2001),
Pot1,
the putative telomere end-binding protein in fission yeast and humans,
Science 2001, 292:1171-1175.
Fairall L, Chapman L, Moss H, de Lange T, Rhodes D (2001),
Structure
of the TRFH dimerization domain of the human telomeric proteins TRF1 and TRF2,
Mol Cell 2001, 8:351-361.
Stansel RM, de Lange T, Griffith JD (2001),
T-loop
assembly in vitro involves binding of TRF2 near the 3' telomeric overhang,
EMBO Jouranal 2001, 20:5532-5540.
Blackburn EH (2001),
Switching
and signaling at the telomere,
Cell 2001, 106:661-673.
Wright WE, Shay JW (2001),
Cellular
senescence as a tumor-protection mechanism: the essential role of counting,
Curr Opin Genet Dev 2001, 11:98-103.
Zhou XZ, Lu KP (2001),
The
Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor,
Cell 2001, 107:347-359.
Pennock, E., K. Buckley, and V. Lundblad (2001),
Cdc13
delivers separate complexes to the telomere for end protection and replication,
Cell 104:387-396.
Kaminker PG, Kim S-H, Taylor RD, Zebarjadian Y, Funk WD, Morin GB, Yaswen P, Campisi J (2001),
TANK2,
a new TRF1-associated PARP, causes rapid induction of cell death upon overexpression,
J Biol Chem 276: 35891–35899. Check: TANK2 = Tankyrase 2.
Moshous, D., Callebaut, I., de Chasseval, R., Comeo, B. Cavez-zana-Calvo, M.,
Le Deist, F., Tezcan, I., Sanal, O., Bertrand, Y., Philippe, N., et al. (2001),
Artemis,
a novel DNA double-strand break repair/V(D)J recombination protein, is mutated in human severe combined immune deficiency,
Cell 105, 177-186.
Bailey, S.M., Comforth M.N., Kurimasa, A., Chen, D.J., and Goodwin, E.H. (2001),
Strand-specific
postreplicative processing of mammalian telomeres,
Science, 293, 2462-2465.
de Lange, T. (2001),
Telomere capping -
one strand fits all,
Science, 292, 1075-1076.
Espejel, S et al. (2002),
Mammalian
KU86 mediates chromosomal fusions and apoptosis caused by critically short telomeres,
EMBO Journal 21, 2207-2219.
Smogorzewska, A., Karlsreder, J., Holtgreve-Grez, H., Jauch, A., and de Lange, T. (2002),
DNA
ligase IV-dependent NHEJ of deprotected mammalian telomeres in G1 and G2,
Current Biology 12: 1635-1644.
Ancelin, K., et al. (2002),
Targeting
assay to study the cis functions of human telomeric proteins:
evidence for the inibition of telomerase by TRF1 and and activation of telomere degradation by TRF2,
Mol. Cell. Biol. 22, 3474-3487.
Jörg F. Rippmann, Klaus Damma and Andreas Schnapp (2002),
Functional
Characterization of the Poly(ADP-ribose) Polymerase Activity of Tankyrase 1, a Potential Regulator of Telomere Length,
Journal of Molecular Biology Volume 323, Issue 2, 18 October 2002, Pages 217-224.
Marsischky, G.T., Wilson, B.A. and Collier, R.J. (2003),
Role
of glutamic acid of human poly-ADP-ribose polymerase in polymer formation.
Evidence for active site similarities to the ADP-ribosylating toxins,
J. Biol. Chem. 270, 3247-3254.
Cook, B. D., J. N. Dynek, W. Chang, G. Shostak, and S. Smith (2002),
Role
for the related poly(ADP-Ribose) polymerases tankyrase 1 and 2 at human telomeres,
Mol. Cell. Biol, 22:332-342. See Tankyrase 1.
Baumann, P., E. Podell, and T. R. Cech (2002),
Human
Pot1 (protection of telomeres) protein: cytolocalization, gene structure, and alternative splicing,
Mol. Cell. Biol. 22:8079-8087.
Granger, M.P., Wright W.E., and Shay, J.W. (2002),
Telomerase in cancer and aging,
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Paulmurugan, R., Umezawa, Y., and Gambhir, S.S. (2002),
Noninvasive
imaging of protein-protein interactions in living subjects by using reporter protein
complementation and reconstitution strategies,
Proc Natl Acad Sci USA 99, 15608-15613.
Stavropoulos, D.J., Bradshaw, P.S., Li, X., Pasic, I., Truong, K.,
Ikura, M., Ungrin, M., and Meyn, M.S. (2002),
The
Bloom syndrome helicase BLM interacts with TRF2 in ALT cells and promotes telomeric DNA synthesis,
Human Molecular Genetics 11, 3135-3144.
Opresko PL, von Kobbe C, Laine JP, Harrigan J, Hickson ID, Bohr VA (2002),
Telomere-binding
protein TRF2 binds to and stimulates the Werner and Bloom syndrome helicases,
J Biol Chem 2002, 277:41110-41119.
Nakamura M, Zhou XZ, Kishi S, Lu KP (2002),
Involvement
of the telomeric protein Pin2/TRF1 in the regulation of the mitotic spindle,
FEBS Letters 2002, 514:193-198.
Cong YS, Wright WE, Shay JW (2002),
Human
telomerase and its regulation,
Microbiol Mol Biol Rev 2002, 66:407-425.
Maser RS, DePinho RA (2002),
Connecting
chromosomes, crisis, and cancer,
Science 2002, 297:565-569.
Kim SH, Kaminker P, Campisi J (2002),
Telomeres,
aging and cancer: in search of a happy ending,
Oncogene 2002, 21:503-511.
de Lange (2002),
Protection
of mammalian telomeres,
Oncogene, 21: 532-540.
Collins, K., and Mitchell, J.R. (2002),
Telomerase in the human organism,
Oncogene 21, 564-579.
Hu, C.D., Chinenov, Y., and Kerppola, T.K. (2002),
Visualization
of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation,
Mol Cell 9, 789-798.
Iwano, T., Tachibana, M, Reth, M., and Shinkai, Y. (2003),
Importance
of TRF1 for Functional Telomere Structure,
Journal of Biological Chemistry 279, 1442-1448 (2003).
d'Adda di Fagagna, F., P. M. Reaper, L. Clay-Farrace, H. Fiegler, P. Carr, T. Von Zglinicki,
G. Saretzki, N. P. Carter, and S. P. Jackson (2003),
A
DNA damage checkpoint response in telomere-initiated senescence,
Nature 426:194-198.
Takai, H., Smogorzewska, A., and de Lange T. (2003),
DNA
damage foci at dysfunctional telomeres,
Current Biology 13, 1549-1556.
Hu, C.D., and Kerppola, T.K. (2003),
Simultaneous
visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis,
Nature Biotechnology 21, 539-545.
Loayza D, de Lange T (2003),
POT1
as a terminal transducer of TRF1 telomere length control,
Nature 2003, 424:1013-1018.
Takai, H., Smogorzewska, A., de Lange, T. (2003),
DNA
damage foci at dysfunctional telomeres,
Current Biology 13: 1549-1556.
Chang W, Dynek JN, Smith S (2003),
TRF1
is degraded by ubiquitin-mediated proteolysis after release from telomeres,
Genes and Development 2003, 17:1328-1333.
Li B, de Lange T (2003),
Rap1
affects the length and heterogeneity of human telomeres,
Mol Biol Cell, 2003, 14:5060-5068.
Lei M, Podell ER, Baumann P, Cech TR (2003),
DNA
self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA,
Nature 2003, 426:198-203.
Zhu XD, Niedernhofer L, Kuster B, Mann M, Hoeijmakers JH, de Lange T (2003),
ERCC1/XPF
removes the 3' overhang from uncapped telomeres and represses formation of telomeric DNA-containing double minute chromosomes,
Mol Cell 2003, 12:1489-1498.
Zou L, Elledge SJ (2003),
Sensing
DNA damage through ATRIP recognition of RPA-ssDNA complexes,
Science 2003, 300:1542-1548.
Deng Z, Atanasiu C, Burg JS, Broccoli D, Lieberman PM (2003),
Telomere
repeat binding factors TRF1, TRF2, and hRAP1 modulate replication of Epstein-Barr virus OriP,
J Virol 2003, 77:11992-12001.
Karlseder, J., Kachatrian, L., Takai, H., Mercer, K., Hingorani, S., Jacks, T., and de Lange, T. (2003),
Targeted
deletion reveals an essential function for the telomere length regulator TRF1,
Mol Cell Biol 23, 6533-6541.
De Rycker M, Venkatesan RN, Wei C, Price CM (2003),
Vertebrate
tankyrase domain structure and sterile-alpha motif (SAM) mediated multimerization,
Biochem J 372: 87–96.
Bai,Y., and Murnane, J.P. (2003),
Telomere
instability in a human tumor cell line expressing a dominant-negative WRN protein,
Human Genetics 113, 337-347.
Karlsreder, J., et al. (2003),
Targeted
deletion reveals an essential function for the telomere length regulator TRF1,
Mol. Cell. Biol. 23, 6533-6541 (2003).
Tarsounas, M., Munoz, P., Claas, A., Smiraldo, P.G., Pittman, D.L.,
Blasco, M.A., and West, S.C. (2004),
Telomere
maintenance requires the RAD51D recombination/repair protein,
Cell 117, 337-347.
Smogorzewska, A., and de Lange, T. (2004),
Regulation
of telomerase by telomeric proteins,
Annual Review of Biochemistry 73, 177-208.
Undarmaa,B., Kodama, S., Suzuki, K., Niwa, O., and Watanabe, M. (2004),
X-ray
induced telomeric instability in Atm-deficient mouse cells,
Biochem. Biophys. Res. Commun. 315, 51-58.
De Rycker M, Price CM (2004),
Tankyrase
polymerization is controlled by its sterile alpha motif and poly(ADP-ribose) polymerase domains,
Mol Cell Biol 24: 9802–9812.
Gelmini S, Poggesi M, Distante V, Bianchi S, Simi L, Luconi M, Raggi CC, Cataliotti L, Pazzagli M, Orlando C (2004),
Tankyrase,
a positive regulator of telomere elongation, is over expressed in human breast cancer,
Cancer Letters 216: 81–87.
[ Links/tankyrase 1 inhibitors,
Images,
Papers,
Patents,
Books].
Armbruster, B. N., C. M. Linardic, T. Veldman, N. P. Bansal, D. L. Downie, and C. M. Counter (2004),
Rescue
of an hTERT mutant defective in telomere elongation by fusion with hPot1,
Mol. Cell. Biol. 24:3552-3561.
Wilson, C.G., Maliery, T.J., and Regan, L. (2004),
Detecting
protein-protein interactions with GPF-fragment reassembly,
Nat Methods 1, 255-262.
Wong, J.M. and Collins, K. (2004),
Telomere
Maintenance and Disease,
Lancet, 362: 983-988.
Tarsounas, M., Munoz, P., Claas, A., Smiraldo, P.G., Pittman, D.L.,
Blasco, M.A., and West, S.C. (2004),
Telomere
maintenance requires the RAD51 recombination repair protein,
Cell 117, 337-347.
Bochkarev A, Bochkareva E (2004),
From
RPA to BRCA2: lessons from single-stranded DNA binding by the OB-fold,
Curr Opin Struct Biol 2004, 14:36-42.
O'Connor MS, Safari A, Liu D, Qin J, Songyang Z (2004),
The
human Rap1 protein complex and modulation of telomere length,
J Biol Chem, 2004, 279:28585-28591.
Dantzer F, Giraud-Panis MJ, Jaco I, Amé JC, Schultz I, Blasco M, Koering CE,
Gilson E, Ménissier-de Murcia J, de Murcia G, Schreiber V (2004),
Functional
interaction between poly(ADP-Ribose) polymerase 2 (PARP-2) and TRF2: PARP activity negatively regulates TRF2, .
Mol Cell Biol 2004, 24:1595-1607.
Lei M, Podell ER, Cech TR (2004),
Structure
of human POT1 bound to telomeric single-stranded DNA provides a model for chromosome end-protection,
Nat Struct Mol Biol 2004, 11:1223-1229.
Dynek JN, Smith S (2004),
Resolution
of sister telomere association is required for progression through mitosis,
Science 2004, 304:97-100.
Lillard-Wetherell K, Machwe A, Langland GT, Combs KA, Behbehani GK, Schonberg SA,
German J, Turchi JJ, Orren DK, Groden J (2004),
Association
and regulation of the BLM helicase by the telomere proteins TRF1 and TRF2,
Hum Mol Genet 2004, 13:1919-1932.
Kim SH, Beausejour C, Davalos AR, Kaminker P, Heo SJ, Campisi J (2004),
TIN2
mediates functions of TRF2 at human telomeres,
J Biol Chem 2004, 279:43799-43804.
Ye JZ, Donigian JR, Van Overbeek M, Loayza D, Luo Y, Krutchinsky AN, Chait BT, De Lange T (2004),
TIN2
binds TRF1 and TRF2 simultaneously and stabilizes the TRF2 complex on telomeres,
J Biol Chem 2004, 279:47264-47271.
Ye J.Z., and de Lange, T. (2004),
TIN2
is a tankyrase 1 PARP modulator in the TRF1 telomere length control complex,
Nature Genetics, 36, 618-623.
d'Adda di Fagagna, SH Teo, SP Jackson (2004),
Functional
links between telomeres and proteins of the DNA-damage response,
[ direct link]
Genes & Development,
18:1781-1799, 2004.
Liu D, O'Connor MS, Qin J, Songyang Z (2004),
Telosome,
a mammalian telomere-associated complex formed by multiple telomeric proteins,
Journal of Biological Chemistry 2004, 279:51338-51342.
Houghtaling BR, Cuttonaro L, Chang W, Smith S (2004),
A
Dynamic Molecular Link between the Telomere Length Regulator TRF1 and the Chromosome End Protector TRF2,
Current Biology, 2004, 14:1621-1631.
Ye JZ, Hockemeyer D, Krutchinsky AN, Loayza D, Hooper SM, Chait BT, de Lange T (2004),
POT1-interacting
protein PIP1: a telomere length regulator that recruits POT1 to the TIN2/TRF1 complex,
Genes and Development, 2004, 18:1649-1654. PIP1 is an alias for TPP1.
Liu D, Safari A, O'Connor MS, Chan DW, Laegeler A, Qin J, Songyang Z (2004),
PTOP
interacts with POT1 and regulates its localization to telomeres,
Nat Cell Biol 2004, 6:673-680. PTOP is an alias for TPP1.
Smogorzewska A, de Lange T (2004),
Regulation
of telomerase by telomeric proteins,
Annual Review of Biochemistry, 73: 177-208.
Seimiya H, Muramatsu Y, Smith S, Tsuruo T (2004),
Functional
subdomain in the ankyrin domain of tankyrase 1 required for poly(ADP-ribosyl)ation of TRF1
and telomere elongation,
Mol Cell Biol 24: 1944–1955.
Wang RC, Smogorzewska A, de Lange T (2004),
Homologous
recombination generates T-loop-sized deletions at human telomeres.
Cell 2004, 119:355-368.
Ye, J. Z.-S., Hockemeyer, D., Krutchinsky, A. N., Loayza, D., Hooper, S. M., Chait, B. T., de Lange, T. (2004).
POT1-interacting
protein PIP1: a telomere length regulator that recruits POT1 to the TIN2/TRF1 complex,
Genes and Development
18: 1649-1654. PIP1 is an alias for TPP1.
Du, X, Shen, J, Kugan, N, Furth, E.E., Lombard, D.B., Cheung, C,
Pak, S, Luo, G, Pignolo, R.J, DePinho, R.A., Guarente, L, and Johnson, F.B. (2004),
Telomere
shortening exposes functions for the mouse Werner and Bloom syndrome genes,
Mol Cell Biol, 24: 8437-8446.
Chang, S., Multani, A.S., Cabrera, N.G., Naylor, M.L., Laud, P., Lombard, D., Pathak, S.,
Guarente, L., and DePinho, R.A. (2004),
Essential
role of limiting telomeres in the pathogenesis of Werner syndrome,
Nature Genetics 36, 877-882, Epub 2004 July 2004.
Li, B., Navarro, S., Kasahara, N., and Comai, L. (2004),
Identification
and biochemical characterization of a Werner's syndrome protein complex with Ku70/80 and poly(ADP-ribose) polymerase-1,
Journal of Biological Chemistry 279, 13659-13667.
Opresko, P.L., Otterlei, M., Graakjaer, J., Bruheim, P.
Dawut, L, Kolvraa, S., May, A., Seidmann, M.M., and Bohr, V.A. (2004),
The
Werner Syndrome Helicase and Exonuclease Cooperate to Resolve Telomeric D Loops in a Manner Regulated by TRF1 and TRF2,
Mol Cell 14, 763-774.
Loayza, D., H. Parsons, J. Donigian, K. Hoke, and T. de Lange (2004),
DNA
binding features of human POT1: a nonamer 5'-TAGGGTTAG-3' minimal binding site, sequence specificity, and internal binding to multimeric sites,
Journal of Biological Chemistry, 279:13241-13248.
Veldman, T., K. T. Etheridge, and C. M. Counter (2004),
Loss
of hPot1 function leads to telomere instability and a cut-like phenotype,
Curr. Biol, 14:2264-2270.
Crabbe, L, Verdun, R.E., Haggblom, C.I., and Karlsreder, J. (2004),
Defective
telomere lagging strand synthesis in cells lacking WRN helicase activity,
Science 306, 1951-1953.
Hanaoka, S., Nagaoi, A., and Nishimura, Y. (2005),
Comparison
between TRF2 and TRF1 of their telomeric DNA-bound structures and DNA-binding activities,
Protein Science 14: 199-130.
Court, R., Chapman, L., Fairall, L., and Rhodes, D. (2005),
How
the human telomeric proteins TRF1 and TRF2 recognize telomeric DNA:
A view from high-resolution crystal structures,
EMBO Rep. 6: 39-45.
Gang Liu, June Chunqiu Hou, Robert T. Watson, and Jeffrey E. Pessin (2005),
Initial
entry of IRAP into the insulin-responsive storage compartment occurs prior to basal or insulin-stimulated plasma membrane recycling,
American Jounal of Physiology Endocrinology and Metabolism November 2005, vol. 289, no. 5, E746-E752.
Tankyrase 1 interacts with IRAP.
Yang, Q., Zheng, Y.L., and Harris C.C. (2005),
POT1
and TRF2 cooperate to maintain telomeric integrity,
Mol Cell Biol, 25, 1070-1080.
Bunch, J. T., N. S. Bae, J. Leonardi, and P. Baumann (2005),
Distinct
requirements for pot1 in limiting telomere length and maintaining chromosome stability,
Mol. Cell. Biol, 25:5567-5578.
Yang, Q., Zheng, Y.L., and Harris, C.C. (2005),
POT1
and TRF2 Cooperate to Maintain Telomeric Integrity,
Mol Cell Biol 25, 1070-1080.
Opresko, P. L., Mason, P. A., Podell, E. R., Lei, M., Hickson, I. D., Cech, T. R., Bohr, V. A. (2005),
POT1
Stimulates RecQ Helicases WRN and BLM to Unwind Telomeric DNA Substrates,
Journal of Biological Chemistry
280: 32069-32080.
Shakirov, E. V., Surovtseva, Y. V., Osbun, N., Shippen, D. E. (2005),
The
Arabidopsis Pot1 and Pot2 Proteins Function in Telomere Length Homeostasis and Chromosome End Protection,
Molecular and Cellular Biology,
25: 7725-7733.
de Lange T (2005),
Shelterin:
the protein complex that shapes and safeguards human telomeres,
Genes and Development, 2005, 19:2100-2110.
Chang P, Coughlin M, Mitchison TJ (2005),
Tankyrase-1
polymerization of poly(ADP-ribose) is required for spindle structure and function,
Nat Cell Biol 2005, 7:1133-1139. See TA/Tankyrase.
Bradshaw PS, Stavropoulos DJ, Meyn MS (2005),
Human
telomeric protein TRF2 associates with genomic double-strand breaks as an early response to DNA damage,
Nature Genetics 2005, 37:193-197.
Zaug AJ, Podell ER, Cech TR (2005),
Human
POT1 disrupts telomeric G-quadruplexes allowing telomerase extension in vitro,
Proc Natl Acad Sci USA 2005, 102:10864-10869.
Bradshaw PS, Stavropoulos DJ, Meyn MS (2005),
Human
telomeric protein TRF2 associates with genomic double-strand breaks as an early response to DNA damage,
Nature Genetics 2005, 37:193-197.
Kaminker P, Plachot C, Kim SH, Chung P, Crippen D, Petersen OW, Bissell MJ, Campisi J, Lelievre SA (2005),
Higher-order
nuclear organization in growth arrest of human mammary epithelial cells:
a novel role for telomere-associated protein TIN2,
J Cell Sci 2005, 118:1321-1330.
Verdun RE, Crabbe L, Haggblom C, Karlseder J (2005),
Functional
human telomeres are recognized as DNA damage in G2 of the cell cycle,
Mol Cell 2005, 20:551-561.
Shakirov EV, Surovtseva YV, Osbun N, Shippen DE (2005),
The
Arabidopsis Pot1 and Pot2 proteins function in telomere length homeostasis and chromosome end protection,
Mol Cell Biol 2005, 25:7725-7733.
Wright, W.E., and Shay, J.W. (2005),
Telomere-binding
factors and general DNA repair,
Nature Genetics, 37: 116-118.
Kelleher, C., I. Kurth, and J. Lingner (2005),
Human
protection of telomeres 1 (POT1) is a negative regulator of telomerase activity in vitro,
Mol. Cell. Biol, 25:808-818.
Lei, M., A. J. Zaug, E. R. Podell, and T. R. Cech. (2005),
Switching
human telomerase on and off with hPOT1 protein in vitro,
Journal of Biological Chemistry, 280:20449-20456.
Hiroyuki Seimiya, Yukiko Muramatsu, Tomokazu Ohishi, and Takashi Tsuruo (2005),
Tankyrase 1
as a target for telomere-directed molecular cancer therapeutics,
Cancer Cell, Vol.7, issue 1, January 2005, pp.25-37.
See also TA/Tankyrase.
Hockemeyer, D., Sfeir, A.J., Shaw, J.W., Wright, W.E., and de Lange, T. (2005),
POT1
protects telomeres from a transient DNA damage response and determines how human chromosomes end,
EMBO Journal 24, 2667-2678.
Celli, G.B., and de Lange, T. (2005),
DNA
processing not required for ATM-mediated telomere damage response after TRF2 deletion,
Nature Cell Biology 7, 712-718.
Rhodes, D. (2005),
The
structural biology of telomeres,
In Telomeres
(eds de Lange, T. et al.) pp. 317-344.
Cold Spring Harbor Laboratory Press, Cold Spring Harbor,N.Y.
Miller, K.M., Rog, O., and Cooper, J.P. (2006),
Semiconservative
DNA replication through telomeres requires TAZ1,
Nature, 440, 824-828. Taz1 is a fission yeast telomeric protein.
Songyang, Z. and Liu, D. (2006)
Inside
the mammalian telomere interactome: regulation and regulatory activities of telomeres,
Critical Review of Eucaryotic Gene Expression, 16, 103-118.
O'Connor, M.S., Safari, A., Xin, H., Liu, D., and Songyang, Z. (2006),
A
critial role for TPP1 and TIN2 interaction in higher-order telomeric complex assembly,
PNAS USA 103, 11874-11879.
Dimitrova, N. and de Lange, T. (2006),
MDC1 accelerates non-homologous end-joining of dysfunctional telomeres,
Genes and Development 20: 3238-3243.
H. Seimiya (2006),
The
telomeric PARP, tankyrases, as targets for cancer therapy,
British Journal of Cancer (2006) 94, 341–345. Describes
tankyrase 1 phosphorylation in insulin-stimulated
cells, ect.
Celli, G.B., Lazzerini Denchi, E., and de Lange, T. (2006),
Ku70
stimulates fusion of dysfunctional telomeres yet protects chromosome ends from homologous recombination,
Nature Cell Biology.
Freibaum, B. D., and C. M. Counter (2006),
hSnm1B
is a novel telomere-associated protein,
Journal of Biological Chemistry, 281:15033-15036.
Hockemeyer D, Daniels JP, Takai H, de Lange T (2006),
Recent
expansion of the telomeric complex in rodents: Two distinct POT1 proteins protect mouse telomeres,
Cell 2006, 126:63-77.
Wu L, Multani AS, He H, Cosme-Blanco W, Deng Y, Deng JM, Bachilo O,
Pathak S, Tahara H, Bailey SM, Deng Y, Behringer RR, Chang S (2006),
Pot1
deficiency initiates DNA damage checkpoint activation and aberrant homologous recombination at telomeres,
Cell 2006, 126:49-62.
Churikov D, Wei C, Price CM (2006),
Vertebrate
POT1 restricts G-overhang length and prevents activation of a telomeric DNA damage checkpoint but is dispensable for overhang protection,
Mol Cell Biol 2006, 26:6971-6982.
Gomez M, Wu J, Schreiber V, Dunlap J, Dantzer F, Wang Y, Liu Y (2006),
PARP1
Is a TRF2-associated Poly(ADP-Ribose)Polymerase and Protects Eroded Telomeres,
Mol Biol Cell 2006, 17:1686-1696.
Verdun RE, Karlseder J (2006),
The
DNA damage machinery and homologous recombination pathway act consecutively to protect human telomeres,
Cell 2006, 127:709-720.
Zhu Q, Yasumoto H, Tsai RY (2006),
Nucleostemin
Delays Cellular Senescence and Negatively Regulates TRF1 Protein Stability,
Mol Cell Biol 2006, 26:9279-9290.
Lee TH, Perrem K, Harper JW, Lu KP, Zhou XZ (2006),
The
F-box protein FBX4 targets PIN2/TRF1 for ubiquitin-mediated degradation and regulates telomere maintenance,
J Biol Chem 2006, 281:759-768.
Ling Wu, Asha S. Multani, Hua He, Wilfredo Cosme-Blanco, Yu Deng, Jian Min Deng,
Olga Bachilo, Sen Pathak, Hidetoshi Tahara, Susan M. Bailey, Yibin Deng, Richard R. Behringer
and Sandy Chang (2006),
Pot1 Deficiency Initiates DNA Damage Checkpoint Activation and Aberrant Homologous Recombination at Telomeres,
Cell, Volume 126, Issue 1, 14 July 2006, Pages 49-62.
Kristine Kelly, (2006),
Evidence of rapid evolution is found
at the tips of chromosomes,
Rockefeller Newswire.
Songyang Z, Liu D (2006),
Inside
the mammalian telomere interactome: regulation and regulatory activities of telomeres,
Crit Rev Eukaryot Gene Expr 2006, 16:103-118.
Fouche N, Cesare AJ, Willcox S, Ozgur S, Compton SA, Griffith JD (2006),
The
basic domain of TRF2 directs binding to DNA junctions irrespective of the presence of TTAGGG repeats,
J Biol Chem 2006, 281:37486-37495.
Baumann P (2006),
Are
mouse telomeres going to pot?,
Cell 2006, 126:33-36.
Atanasiu C, Deng Z, Wiedmer A, Norseen J, Lieberman PM (2006),
ORC
binding to TRF2 stimulates OriP replication,
EMBO Rep 2006, 7:716-721.
O'Connor MS, Safari A, Xin H, Liu D, Songyang Z (2006),
A
critical role for TPP1 and TIN2 interaction in high-order telomeric complex assembly,
Proc Natl Acad Sci USA 2006, 103:11874-11879.
He H, Multani AS, Cosme-Blanco W, Tahara H, Ma J, Pathak S, Deng Y, Chang S (2006),
POT1b
protects telomeres from end-to-end chromosomal fusions and aberrant...
(mouse genome; code-scrambled reference, scrambled part removed).
See Zhong Deng, Jayaraju Dheekollu, Dominique Broccoli, Anindya Dutta, and Paul Lieberman (2007),
The
Origin Recognition Complex Localizes to Telomere Repeats and Prevents Telomere-Circle Formation,
Current Biology 17, Nov.20,2007.
Denchi, E.L., and de Lange, T. (2007),
Protection
of telomeres through independent control of ATM and ATR by TRF2 and POT1,
Nature 448, 1068-1071.
Songyang Z (2007),
TPP1
is a homologue of ciliate TEBP-beta and interacts with POT1 to recruit telomerase,
Nature 2007, 445:559-562.
Wang F, Podell ER, Zaug AJ, Yang Y, Baciu P, Cech TR, Lei M (2007),
The
POT1-TPP1 telomere complex is a telomerase processivity factor,
Nature 2007, 445:506-510.
Potts PR, Yu H (2007),
The
SMC5/6 complex maintains telomere length in ALT cancer cells through SUMOylation of telomere-binding proteins,
Nat Struct Mol Biol 2007, 14:581-590.
Wu Y, Xiao S, Zhu XD (2007),
MRE11-RAD50-NBS1
and ATM function as co-mediators of TRF1 in telomere length control,
Nat Struct Mol Biol 2007, 14:832-840.
Blasco MA (2007),
The
epigenetic regulation of mammalian telomeres,
Nat Rev Genet 2007, 8:299-309.
Verdun RE, Karlseder J (2007),
Replication
and protection of telomeres,
Nature 2007, 447:924-931.
Amiard S, Doudeau M, Pinte S, Poulet A, Lenain C, Faivre-Moskalenko C,
Angelov D, Hug N, Vindigni A, Bouvet P, Paoletti J, Gilson E, Giraud-Panis MJ (2007),
A topological mechanism for TRF2-enhanced strand invasion,
Nat Struct Mol Biol 2007, 14:147-154.
Hiroshi Aoki, Eiji Iwado, Mark S. Eller, Yasuko Kondo, Keishi Fujiwara, Guang-Zhi Li, Kenneth R. Hess,
Doris R. Siwak, Raymond Sawaya, Gordon B. Mills, Barbara A. Gilchrest and Seiji Kondo (2007).
Telomere 3' overhang-specific DNA oligonucleotides
induce autophagy in malignant glioma cells,
The FASEB Journal 2007;21:2918-2930.
Qin Yang, Ran Zhang, Izumi Horikawa, Kaori Fujita, Yalda Afshar,
Antti Kokko, P ivi Laiho, Lauri A. Aaltonen and Curtis C. Harris (2007),
Functional Diversity of
Human Protection of Telomeres 1 Isoforms in Telomere Protection and Cellular Senescence,
Cancer Research
67, 11677-11686, December 15, 2007.
Martin V, Du LL, Rozenzhak S, Russell P (2007),
Protection
of telomeres by a conserved Stn1-Ten1 complex,
Proc Natl Acad Sci USA 2007, 104:14038-14043.
Gao H, Cervantes RB, Mandell EK, Otero JH, Lundblad V (2007),
RPA-like
proteins mediate yeast telomere function,
Nat Struct Mol Biol 2007, 14:208-214.
Chen LY, Liu D, Songyang Z (2007):
Telomere
maintenance through spatial control of telomeric proteins,
Mol Cell Biol 2007, 27:5898-5909.
Lamesh, P., Li, N., Milstein, S., Fan, C., Hao, T., Szabo, G., Hu, Z., Venkatesan, K., Bethel, G., Martin, P.,
Rogers, J., Lawlor, s., McLaren, S., Dicrot, A., Borick, H., Cusick, M.E., Vandenhaute, J.,
Dunham, I., Hill, D.E., and Vidal, M. (2007),
hORFeome v3.1:
a resource of human open reading frames representing over 10,000 human genes,
Genomics 89, 307-315.
Canudas, S., Houghtaling, B.R., Kim, J.Y., Dynek, J.N., Chang, W.G., and Smith, S. (2007),
Protein
requirements for sister telomere association in human cells,
EMBO Journal 26, 4867-4878.
Hockemeyer, D., W. Palm, T. Else, J. P. Daniels, K. K. Takai, J. Z. Ye, C. E. Keegan,
T. de Lange, and G. D. Hammer (2007),
Telomere
protection by mammalian Pot1 requires interaction with Tpp1,
Nature Structural and Molecular Biology, 14:754-761.
Xin, H., Liu, D., Wan, M., Safari, A., Kim, H., Sun, W., O'Connor M, S., and Songyang, Z. (2007),
TPP1
is a homologue of ciliate TEBP-beta and interacts with POT1 to recruit telomerase,
Nature 445, 559-562.
Else, T., et al. (2008),
TPP1/Acd
maintains genomic stability through a complex role in telomere protection,
Chromosome Research 15, 1001-1013.
Xin, H, Liu, D, Songyang, Z (2008),
The
telosome/shelterin complex and its functions,
Genome Biology 9: 232.
Etheridge, K. T., S. A. Compton, K. S. Barrientos, S. Ozgur, J. D. Griffith, and C. M. Counter (2008),
Tethering
telomeric double- and single-stranded DNA-binding proteins inhibits telomere elongation,
Journal of Biological Chemistry, 283:6935-6941.
Barrientos, K. S., M. F. Kendellen, B. D. Freibaum, B. N. Armbruster, K. T. Etheridge, and C. M. Counter (2008),
Distinct functions of POT1 at telomeres,
Mol. Cell. Biol, 28:5251-5264.
Hu CD, Kerppola TK (2008),
Simultaneous
visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis,
Nature Biotechnology 2003, 21:539-545.
Miyoshi T, Kanoh J, Saito M, Ishikawa F (2008),
Fission
yeast Pot1-Tpp1 protects telomeres and regulates telomere length,
Science 2008, 320:1341-1344.
Lee J, Mandell EK, Tucey TM, Morris DK, Lundblad V (2008),
The
Est3 protein associates with yeast telomerase through an OB-fold domain,
Nature Structural and Molecular Biology 2008, 15:990-997.
Young Yu E, Wang F, Lei M, Lue NF (2008),
A
proposed OB-fold with a protein-interaction surface in Candida albicans telomerase protein Est3,
Nat Structural and Molecular Biology 2008, 15:985-989.
Longhese MP (2008),
DNA
damage response at functional and dysfunctional telomeres,
Genes and Developement 2008, 22:125-140.
Chen Y, Yang Y, van Overbeek M, Donigian JR, Baciu P, de Lange T, Lei M (2008),
A
Shared Docking Motif in TRF1 and TRF2 Used for Differential Recruitment of Telomeric Proteins,
Science 2008, 319:1092-1096.
Tarassov, K., Messier, V., Landry, C.R., Radinovic, S., Serna Molina, M.M., Shames, I.,
Malitskaya, Y., Vogel, J., Bussey, H., and Michnick, S.W. (2008),
An
in vivo map of the yeast protein interactome,
Science 320, 1465-1470.
Kim, S.H., Davalos, A.R., Heo, S.J., Rodier, F., Zou, Y.,
Beausejour, C., Kaminker, P., Yannone, S.M., and Campisi, J. (2008),
Telomere
dysfunction and cell survival: roles for distinct TIN2-containing complexes,
Journal of Cell Biology 181, 447-460.
Tsangarts, E., et. al, (2008),
Ataxia
and pancytopenia caused by a mutation in TINF2,
Human Genetics, 124, 507-513.
Raynaud, C. M., L. Sabatier, O. Philipot, K. A. Olaussen, and J. C. Soria (2008),
Telomere
length, telomeric proteins and genomic instability during the multistep carcinogenic process,
Crit. Rev. Oncol. Hematol, 66:99-117.
Palm, W. and de Lange, T. (2008),
How
shelterin protects mammalian telomeres,
Annual Review of Genetics 42, 301-334.
Agnel Sfeir, Settapong T. Kosiyatrakul, Dirk Hockemeyer, Sheila L. MacRae, Jan Karlseder,
Carl L. Schildkraut and Titia de Lange (2009),
Mammalian
Telomeres Resemble Fragile Sites and Require TRF1 for Efficient Replication,
Cell 138, 90-103, July 10, 2009.
Kim, H., Lee, O.H., Xin, H., Chen, L.Y., Qin, J.,
Chae, H.K., Lin, S.Y., Safari, A., Liu, D., and Songyang, Z. (2009),
TRF2
functions as a protein hub and regulates telomere maintenance by recognizing specific peptide motifs,
Nature Structural Molecular Biology 16, 372-379.
Peng, G., Lin, S.Y. (2009),
BRIT1/MCPH1
is a multifunctional DNA damage responsive protein mediating DNA repair-associated chromatin remodeling,
Cell Cycle 8.
Kendellen, M. F., Barrientos, K. S., Counter, C. M. (2009),
POT1
Association with TRF2 Regulates Telomere Length,
Mol. Cell. Biol. 29: 5611-5619.
Oganesian, L., Karlseder, J. (2009),
Telomeric
armor: the layers of end protection,
Journal of Cell Science
122: 4013-4025.
Mitchell, T. R. H., Glenfield, K., Jeyanthan, K., Zhu, X.-D. (2009),
Arginine
Methylation Regulates Telomere Length and Stability,
Mol. Cell. Biol. 29: 4918-4934.
Dejardin, J., and Kingston, R.E. (2009),
Purification
of proteins associated with specific genomic Loci,
Cell 136, 175-186.
Marco Folinia, Paolo Gandellinia and Nadia Zaffaroni (2009),
Targeting the telosome:
Therapeutic implications,
Biochimica et Biophysica Acta,
April 2009, 309-316. See lifexnotes5.html
( Telomeric Proteins Nomenclature) notes.
Kaminker PG, Kim SH, Desprez PY, Campisi J. (2009),
A
novel form of the telomere-associated protein TIN2 localizes to the nuclear matrix,
Cell Cycle 2009: 8: 931–939.
de Lange, T. (2009),
How
telomeres solve the end-protection problem,
Science 326, 948-952.
Abreu, E., Aritonovska, E., Reichenbach, P., Cristofari, G., Culp, B., Terns, R. M., Lingner, J., Terns,
M. P. (2010),
TIN2-Tethered
TPP1 Recruits Human Telomerase to Telomeres In Vivo,
Mol Cell Biol. 30: 2971-2982.
Lee, J., Mandell, E. K., Rao, T., Wuttke, D. S., Lundblad, V. (2010),
Investigating
the role of the Est3 protein in yeast telomere replication.
Nucleic Acids Research
38: 2279-2290.
Ok-Hee Lee, Hyeung Kim, Quanyuan He, Hwa Jin Baek, Dong Yang, Liuh-Yow Chen,
Jiancong Liang, Heekyung Kate Chae, Amin Safari, Dan Liu and Zhou Songyang (2011),
Genome-wide
YFP Fluorescence Complementation Screen Identifies New Regulators for Telomere Signaling in Human Cells,
Molecular
and Cellular Proteomics, 2011, 10, M110.001628.
Paula Martinez and Maria A. Blasco (2010),
Role
of shelterin in cancer and aging,
Aging Cell 9, 653-666.
Paula Martinez and Maria A. Blasco (2011),
Telomeric
and extra-telomeric roles for telomerase and the telomere-binding proteins,
Nature Reviews Cancer, vol. 11, March 2011, 161-176.
Sichun Lin (2011),
Analysis
of the Role of TRF1 and SMG6 in Telomere Length Maintenance, MS Thesis, McMaster University.
[ Index/hEST1A].
Music: Jesse by Carly Simon.
POT1 papers
Ye, J. Z.-S., Hockemeyer, D., Krutchinsky, A. N., Loayza, D., Hooper, S. M., Chait, B. T., de Lange, T. (2004),
POT1-interacting
protein PIP1: a telomere length regulator that recruits POT1 to the TIN2/TRF1 complex,
Genes and Development
18: 1649-1654.
Shakirov, E. V., Surovtseva, Y. V., Osbun, N., Shippen, D. E. (2005),
The
Arabidopsis Pot1 and Pot2 Proteins Function in Telomere Length Homeostasis and Chromosome End Protection,
Molecular and Cellular Biology,
25: 7725-7733.
Opresko, P. L., Mason, P. A., Podell, E. R., Lei, M., Hickson, I. D., Cech, T. R., Bohr, V. A. (2005),
POT1
Stimulates RecQ Helicases WRN and BLM to Unwind Telomeric DNA Substrates,
Journal of Biological Chemistry
280: 32069-32080.
Kendellen, M. F., Barrientos, K. S., Counter, C. M. (2009),
POT1
Association with TRF2 Regulates Telomere Length,
Mol. Cell. Biol. 29: 5611-5619.
________ 7.9 Telomerase Functional Environment
[ Links,
Images,
Video,
Papers,
Books;
Index/Telosome].
J. Roy, T.B. Fulton, and Elizabeth H. Blackburn (1998).
Specific
telomerase RNA residues distant from the template are essential for telomerase function.
Genes and Development 12, 3286-3300.
A. Ray and K.W. Runge (1999).
The
yeast telomere length counting machinery is sensitive to sequences at the telomere-nontelomere junction.
Molecular and Cellular Biology
19, 31-45.
Shawn E. Holt, Dara L. Aisner, Joseph Baur, Valerie M. Tesmer, Marife Dy, Michel Oullette, James B. Trager,
Gregg B. Morin, David O. Toft, Jerry W. Shay, Woodring E. Wright, and Michael A. White (1999),
Functional
requirement of p23 and Hsp90 in telomerase complexes,
Genes and Development 1999 13: 817-826.
J.C. Prescott and Elizabeth H. Blackburn (2000).
Telomerase
template mutations reveal sequence-specific requirements for the activation and repression of telomerase action at telomeres.
Molecular and Cellular Biology 20, 2941-2948.
Etheridge, K.T., S.S.Banik, B.N.Armbruster, Y. Zhu, R. M. Terns, M.P. Turns, and C.M.Counter (2002),
The
nucleolar localization domain of the catalytic unit of human telomerase,
J. Biol. Chem. 15:15.
Brian R. Keppler, Allen T. Grady, and Michael B. Jarstfer (2006),
The
Biochemical Role of the Heat Shock Protein 90 Chaperone Complex in Establishing Human Telomerase Activity,
The Journal of Biological Chemistry 281, No.29, 19840-19848.
Eva Sykorova and Jiri Fajkus (2009),
Structure-Function relationships in Telomerase Genes,
Biol. Cell (2009).101, 375-392.
Venteicher AS, Abreu EB, Meng Z, McCann KE, Terns RM, Veenstra TD, Terns MP, Artandi SE, (2009).
A human telomerase holoenzyme protein
required for Cajal body localization and telomere synthesis,
Science (2009). Identifies the roles of dyskerin
[ Links] and
TCAB1 [ Links] in detailed telomerase activity,
and includes an analysis of other important factors.
________ 7.10 Telomerase and Protection of Mitochondrial DNA
[ Links,
Images,
Video,
Papers,
Books].
Zhang, P., Chan, S.L., Fu, W., Medoza, M., and Mattson, M.P. (2003),
TERT
suppresses apoptosis at a premitochondrial step by a mechanism requiring reverse transcriptase activity
and 14-3-3 protein binding ability,
FASEB Journal 17, 767-769.
Haendeler, J. et al. (2004),
Antioxidants
inhibit nuclear export of telomerase reverse transcriptase and delay replicative senescence of endothelial cells,
Circulation Research 94, 768-775.
Santos, J.H., Meyer, J.N., Skorvaga, M., Annab, L.A., and Van Houten, B. (2004),
Mitochondrial
hTERT exacerbates free-radical mediated mtDNA damage,
Aging Cell 3, 399-411.
Santos, J.H., Meyer, J.N., and Van Houten, B. (2006),
Mitochondrial
localization of telomerase as a determinant for hydrogen peroxide-induced mitochondrial DNA damage and apoptosis,
Human Molecular Genetics 15, 1757-1768.
Massard, C., et al. (2006),
hTERT:
a novel endogenous inhibitor of the mitochondrial cell death pathway,
Oncogene 27, 3754-3760.
Lee, J., et al. (2008),
TERT
promotes cellular and organismal survival independently of telomerase activity,
Oncogene 27, 3754-3760.
Haendeler J, Jakob S, Altschmied J, Goy C, Spyridopoulos I, Zeiher AM, Brandt U, Dimmeler S, et al., (2009),
Mitochondrial telomerase reverse transcriptase [?]
binds to and protects mitochondrial DNA and function from damage,
Arterioscler. Thromb. Vasc. Biol. (2009).
" Mitochondrial TERT
exerts a novel protective function
by binding to mitochondrial DNA, increasing respiratory chain activity and protecting against
oxidative stress-induced damage."
______ 7.11 Measuring Telomere Length
[ lifexlabs/Telomeasures,
LifexLabs/Telomeasures,
Links,
Images,
Video,
Papers,
Books].
_________ 7.11.1 Measuring Telomere Length with PCR Methods (Cawthon).
__________[ LifexLabs/Telomeasures,
Links,
Images,
Video,
Papers,
Books].
Marcel E. Gil and Thérèsa L. Coetzer, (2004).
Real-time quantitative PCR of telomere length,
Molecular Biotechnology,
Volume 27, Number 2, June, 2004.
(2008), Biotechniques.com/A
quantitative real-time PCR method for absolute telomere length.
Richard M. Cawthon (2009),
Telomere
length measurement by a novel monochrome multiplex quantitative PCR method,
Nucleic Acids Research,
2009 February; 37(3)
_________ 7.11.2 Measuring with Southern blot analysis of telomere restriction fragments.
__________[ LifexLabs/Telomeasures,
Links,
Images,
Video,
Papers,
Books].
_________ 7.11.3 Measuring with Quantitative fluorescence in situ hybridization with digital fluorescence microscopy (Q-FISH).
__________[ LifexLabs/Telomeasures,
Links,
Images,
Video,
Papers,
Books].
Canela A, Vera E, Klatt P, Blasco MA (2007),
High-throughput
telomere length quantification by FISH and its application to human population studies,
PNAS USA, 2007 Mar 27; 104(13):5300-5.
_________ 7.11.4 Measuring with Quantitative fluorescence in situ hybridization with flow cytometry (flow-FISH).
__________[ LifexLabs/Telomeasures,
Links,
Images,
Video,
Papers,
Books].
_________ 7.11.5 Measuring with Quantitative fluorescent in situ hybridization laser scanning cytometry (Q-FISHLSC).
__________[ LifexLabs/Telomeasures,
Links,
Images,
Video,
Papers,
Books].
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